CTRP3 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: CTRP3 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-3565
• Supplier Catalog Number: 3565
• Alternative Catalog Number: PRS-3565-0.02,PRS-3565-0.1
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Anti-CTRP3 antibody (3565) was raised against a peptide corresponding to 16 amino acids near the center of human CTRP3. The immunogen is located within amino acids 90-140 of CTRP3.
• Conjugation: Unconjugated
• Alternative Names: CTRP3 Antibody: CORS, CORCS, CTRP3, CORS26, C1ATNF3, CORS-26, UNQ753/PRO1484, Complement C1q tumor necrosis factor-related protein 3, Collagenous repeat-containing sequence 26 kDa protein

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/mL
• Molecular Weight: Predicted: 27kDObserved: 30 kD (Post-modyfication: 1 N-linked glycosylation)
• NCBI: 114899
• UniProt: Q9BXJ4
• Buffer: CTRP3 Antibody is supplied in PBS containing 0.02% sodium azide.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1 µg/mL, IHC: 2 µg/mL, IF: 10 µg/mL.Antibody validated: Western Blot in human, mouse and rat samples, Immunohistochemistry in mouse samples, Immunofluorescence in mouse samples. All other applications and species not yet tested.

Images

Figure 4 Western Blot Validation in Mouse Heart Tissue Lysate in the (A) absence and (B) presence of blocking peptideLoading: 15 &956,g of lysates per lane.Antibodies: CTRP3, 3565 (1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Figure 6 Immunofluorescence Validation of CTRP3 in Mouse Heart TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed mouse heart tissue labeling CTRP3 with 3565 at 10 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Figure 5 Immunohistochemistry Validation of CTRP3 in Mouse Heart Tissue Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-CTRP3 antibody (3565) at 2 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Figure 1 Western Blot Validation in Human and Mouse Cell LinesLoading: 15 &956,g of lysates per lane.Antibodies: CTRP3, 3565 (1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Figure 2 Western Blot Validation with Recombinant ProteinLoading: 30 ng of human CTRP3 recombinant protein per lane.Antibodies: CTRP3, 3565 (2 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Figure 3 Western Blot Validation in Mouse and Rat Tissue LysatesLoading: 15 &956,g of lysates per lane.Antibodies: CTRP3, 3565 (1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.