Produced from sera of rabbits immunized with highly pure recombinant Rat SDF-1 alpha. Anti-Rat SDF-1 alpha-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Rat SDF-1 alpha by sandwich ELISA (using 100 µl/well), a concentration of 0.25 - 1.0 µg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProScis Polyclonal Anti-Rat SDF-1 alpha as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat SDF-1 alpha. Western Blot To detect Rat SDF-1 alpha by Western Blot analysis, this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. When used in conjunction with compatible development reagents, the detection limit for recombinant Rat SDF-1 alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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