Produced from sera of rabbits immunized with highly pure recombinant Human IL-36 gamma. Anti-Human IL-36 gamma-specific antibody was purified by affinity chromatography and then biotinylated.
Conjugation:
Biotin
Clonality:
Polyclonal
Concentration:
batch dependent
Form:
Lyophilized
Application Dilute:
Centrifuge vial prior to opening.
Application Notes:
Sandwich ELISA: To detect Human IL-36 gamma by sandwich ELISA (using 100 ml/well), a concentration of 0.25 - 1.0 µg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProScis Polyclonal Anti- Human IL-36 gamma as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-36 gamma. Western Blot To detect Human IL-36 gamma by Western Blot analysis, this antibody can be used at a concentration of 0.1 - 0.2 mg/ml. When used in conjunction with compatible development reagents, the detection limit for recombinant Human IL-36 gamma is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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