Avian Influenza H5N1 Hemagglutinin (IN2) Antibody, Unconjugated, Goat, Polyclonal

Product Overview

• Article Name: Avian Influenza H5N1 Hemagglutinin (IN2) Antibody, Unconjugated, Goat, Polyclonal
• Biozol Catalog Number: PRS-3913
• Supplier Catalog Number: 3913
• Alternative Catalog Number: PRS-3913-0.02,PRS-3913-0.1
• Manufacturer: ProSci
• Host: Goat
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Virus
• Immunogen: Avian Influenza H5N1 Hemagglutinin (IN2) Antibody was raised against a synthetic peptide corresponding to 10 amino acids near the center of the Hemagglutinin protein.Efforts were made to use relatively conserved regions of the viral sequence as the antigen.The immunogen is located within amino acids 260 - 310 of Avian Influenza H5N1 Hemagglutinin (IN2) Antibody.
• Conjugation: Unconjugated
• Alternative Names: Avian Influenza Hemagglutinin 4 Antibody:

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/mL
• UniProt: Q692M2
• Buffer: Avian Influenza Hemagglutinin 4 Antibody is supplied in PBS containing 0.02% sodium azide.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 2 µg/mL. Avian Influenza Hemagglutinin antibody can be used for the detection of the Hemagglutinin protein from the H5N1 strain of avian influenza A in ELISA and WB. It will detect 10 ng of free peptide at 1 µg/mL. All other applications and species not yet tested.

Images

Figure 2 ELISA Validation with H5N1 HA Protein Coating Antigen: H5N1 Hemagglutinin recombinant protein (Cat. No. 95-101), 2 &956,g/mL, incubated at 4 &730,C overnight. Detection Antibodies: Avian Influenza H5N1 HA antibody, 3913, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Rabbi anti-goat HRP at 1:10,000, incubated at RT for 1 hr.

Figure 1 Western Blot Validation with H5N1 HA Protein Loading: 30 ng H5N1 Hemagglutinin recombinant protein (Cat. No. 95-101). Antibodies: Avian Influenza H5N1 HA antibody, 3913, 1h incubation at RT in 5% NFDM/TBST. Secondary: Rabbit anti-goat IgG HRP conjugate at 1:10,000 dilution. Lane 1: 0.5 &956,g/mL, Lane 2: 1 &956,g/mL