TSLP Antibody, Unconjugated, Rabbit, Polyclonal
Catalog Number:
PRS-4021
- Images (8)
| Article Name: | TSLP Antibody, Unconjugated, Rabbit, Polyclonal |
| Biozol Catalog Number: | PRS-4021 |
| Supplier Catalog Number: | 4021 |
| Alternative Catalog Number: | PRS-4021-0.02,PRS-4021-0.1 |
| Manufacturer: | ProSci |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, IHC-P, WB |
| Species Reactivity: | Human |
| Immunogen: | Anti-TSLP antibody (4021) was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human TSLP. The immunogen is located within the last 50 amino acids of TSLP. |
| Conjugation: | Unconjugated |
| Alternative Names: | TSLP Antibody: Thymic stromal lymphopoietin |
| Application Dilute: | Optimal dilutions for each application to be determined by the researcher. |
| Application Notes: | WB: 0.25 - 2 µg/mL, IHC-P: 2.5 µg/mL.Antibody validated: Western Blot in human samples, Immunohistochemistry and Immunocytochemistry in human samples. All other applications and species not yet tested. |
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Figure 2 Western Blot Validation in Human Jurkat Cell LineLoading: 15 &956,g of lysates per lane.Antibodies: TSLP 4021 (A: 1 &956,g/mL, B: 2 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. |
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Figure 3 Immunohistochemistry Validation of TSLP in Human Brain Tissue Immunohistochemical analysis of paraffin-embedded Human Brain Tissue using anti-TSLP antibody (4021) at 2.5 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. |
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Figure 4 Induced Expression Validation in Human Oral Keratinocytes (Bjerkan et al., 2015) TSLP expression detected by anti-TSLP antibodies (4021) was upregulated in cultured human oral keratinocytes after 24 h in response to poly(I:C), |
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Figure 6 Induced Expression Validation of TSLP in Human Corneal Epithelium (Lin et al., 2018) Immunohistochemical images showing theTSLP protein detected by anti-TSLP antibodies in donor corneal tissues without (Control) or after exposure to IL-32 ex vivo, an isotype IgG antibody was used as a negative control. The production of TSLP was increased after IL-32 treatment. |
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Figure 7 Induced Expression Validation of TSLP in Human Corneal Tissues (Lin et al., 2013) Immunohistochemical images showing TSLP protein detected by anti-TSLP antibodies in ex vivo donor human corneal tissues without (B1) or with exposure to IL-33 (B2). An isotype IgG antibody (B3) was used as a negative control. Magnificaiton 400X. The staining confirms the increased level of TSLP after IL-33 treatment. |
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Figure 8 Induced Expression Validation of TSLP in Mesenchymal Stromal Cells (MSCs). (Allakhverdi et al., 2013) Immunocytochemical images showing TSLP protein detected by anti-TSLP antibodies in BM-MSCs unstimulated (control) or stimulated with IL-1/TNF or supernatants of activated MCs (MCactSNT). Isotype is used as a negative control. The production of TSLP increased with the stimulated conditions. |
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Figure 1 Western Blot Validation with Recombinant ProteinLoading: 30 ng of human TSLP recombinant protein per lane.Antibodies: TSLP 4021, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: 0.25 &956,g/mLLane 2: 0.5 &956,g/mLLane 3: 1 &956,g/mL |
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Figure 5 Regulated Expression Validation of TSLP in COPD Patients. (Anzalone et al., 2018) (A) shows NHBE cells, in the absence (Lane 2) or presence (Lane 3) of Tiotropium , were stimulated with rhIL-17A. (B) shows NHBE cells, with ISs from COPD patients untreated (Lane 2 and Lane 3) or treated (Lane 4) with anti-IL-17A antibody. (A) and (B) show TSLP expression decreases with the treatment of anti-cholinergic drugs. |
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