CREB1 Antibody, Unconjugated, Goat, Polyclonal

Product Overview

• Article Name: CREB1 Antibody, Unconjugated, Goat, Polyclonal
• Biozol Catalog Number: PRS-43-363
• Supplier Catalog Number: 43-363
• Alternative Catalog Number: PRS-43-363-0.1
• Manufacturer: ProSci
• Host: Goat
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human, Mouse
• Immunogen: The immunogen for this antibody is: C-DSQESVDSVTDSQK
• Conjugation: Unconjugated
• Alternative Names: CREB1, cAMP responsive element binding protein 1, CREB, CREB-1, active transcription factor CREB, cAMP-response element-binding protein-1, cAMP-responsive element-binding protein 1, cyclic AMP-responsive element-binding protein 1, transactivator protein

Product Properties

• Clonality: Polyclonal
• Concentration: 500 ug/mL
• Molecular Weight: Approx 45kDa
• NCBI: 1385
• UniProt: P16220
• Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing.
• Form: Liquid

Application Notes

• Application Notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 45kDa band observed in nuclear lysates of cell lines Jurkat and NIH3T3 (calculated MW of 36.7kDa according to NP_604391.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3ug/ml.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Images

43-363 (0.1ug/ml) staining of Jurkat (A) and NIH3T3 (B) nuclear lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.