Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing.
Form:
Liquid
Application Notes:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 100kDa band observed in Mouse and Rat Skeletal Muscle lysates (calculated MW of 96.4kDa according to Mouse NP_031741.1 and 97.0kDa according to Rat NP_068628.1). Recommended concentration: 0.03-0.1ug/ml.
43-364 (0.03ug/ml) staining of Mouse (A) and Rat (B) Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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