Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing.
Form:
Liquid
Application Notes:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 33kDa band observed in Human Colon lysates (calculated MW of 33.3kDa according to NP_001217.2). Recommended concentration: 0.5-1.5ug/ml.
43-366 (0.5ug/ml) staining of Human Colon lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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