Pyruvate Dehydrogenase Peptide

Catalog Number: PRS-45-107P
Article Name: Pyruvate Dehydrogenase Peptide
Biozol Catalog Number: PRS-45-107P
Supplier Catalog Number: 45-107P
Alternative Catalog Number: PRS-45-107P-0.1
Manufacturer: ProSci
Category: Proteine/Peptide
Application: Blocking
Alternative Names: E3BP, OPDX, PDX1, proX, DLDBP, Pyruvate dehydrogenase protein X component, mitochondrial, Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex, E3BP
NCBI: 8050
UniProt: O00330
Buffer: Pyruvate Dehydrogenase peptide is supplied as 100 µg of dried peptide. When the peptide is reconstituted in 200 µL water, the concentration will be 0.5 mg/mL.
Form: Lyophilized
Application Notes: Pyruvate Dehydrogenase Peptide is used for blocking the activity of Pyruvate Dehydrogenase antibody (catalog number 45-107). The Pyruvate Dehydrogenase antibody ships at 0.5 mg/mL and the Pyruvate Dehydrogenase Peptide ships as a 100 ug pellet. When peptide is reconstituted in 200 uL water, the concentration will be 0.5 mg/mL, identical to that of the antibody. The best starting ratio would be 1:1 (which means molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volume of peptide and antibody at the required dilution and leave at ambient temperature for 20 minutes. Then incubate and develop in parallel two blots: one with the antibody and one with the antibody which has been pre-absorbed with the provided peptide.