NPTX2 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: NPTX2 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-4573
• Supplier Catalog Number: 4573
• Alternative Catalog Number: PRS-4573-0.02,PRS-4573-0.1
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Anti-NPTX2 antibody (4573) was raised against a peptide corresponding to 16 amino acids near the center of human NPTX2. The immunogen is located within amino acids 170 - 220 of NPTX2.
• Conjugation: Unconjugated
• Alternative Names: NPTX2 Antibody: NP2, NARP, NP-II, Neuronal pentraxin-2, Neuronal pentraxin II, NP2

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/mL
• Molecular Weight: Predicted: 47kDObserved: 47 kD
• NCBI: 4885
• UniProt: P47972
• Buffer: NPTX2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 0.5-4 µg/mL, IHC: 5 µg/mL, IF: 20 µg/mL.Antibody validated: Western Blot in human, mouse and rat samples, Immunohistochemistry in human and mouse samples, Immunofluorescence in human and mouse samples. All other applications and species not yet tested.

Images

Figure 4 Immunofluorescence Validation of NPTX2 in Human Brain TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed human brain tissue labeling NPTX2 with 4573 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Figure 6 Immunofluorescence Validation of NPTX2 in Mouse Brain TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain issue labeling NPTX2 with 4573 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).

Figure 5 Immunohistochemistry Validation of NPTX2 in Human Brain Immunohistochemical analysis of paraffin-embedded human brain using anti-NPTX2 antibody (4573) at 5 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 &730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Figure 7 Immunohistochemistry Validation of NPTX2 in Mouse Brain Tissue Immunohistochemical analysis of paraffin-embedded mouse brain issue using anti-NPTX2 antibody (4573) at 5 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Figure 1 Western Blot Validation in Mouse Brain Tissue LysateLoading: 15 &956,g of lysates per lane.Antibodies: NPTX2 4573 (A: 0.5 &956,g/mL, B: 1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Figure 3 Western Blot Validation in Mouse Brain Tissue LysateLoading: 15 &956,g of lysates per lane.Antibodies: NPTX2 4573 (1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.A: Absence of blocking peptide B: Presence of blocking peptide.

Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 &956,g of lysates per lane.Antibodies: NPTX2 4573 (2 &956,g/mL), NPTX2 64-234 (4 &956,g/mL), and beta-actin 3779 (1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.