Albumin Antibody, Unconjugated, Gallus, Polyclonal

Product Overview

• Article Name: Albumin Antibody, Unconjugated, Gallus, Polyclonal
• Biozol Catalog Number: PRS-5159
• Supplier Catalog Number: 5159
• Alternative Catalog Number: PRS-5159-0.02,PRS-5159-0.1
• Manufacturer: ProSci
• Host: Gallus
• Category: Antikörper
• Application: ELISA, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Albumin antibody was raised against a 13 amino acid synthetic peptide near the center of human Albumin.The immunogen is located within amino acids 340 - 390 of Albumin.
• Conjugation: Unconjugated
• Alternative Names: Albumin Antibody: PRO0883, PRO0903, PRO1341, Serum albumin

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/mL
• Molecular Weight: Predicted: 69kD Observed: 69 kD
• NCBI: 213
• UniProt: P02768
• Buffer: Albumin Antibody is supplied in PBS containing 0.02% sodium azide.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1-2 µg/mL, IHC-P/IF: 10-20 µg/mL, IHC-P: 2-10 µg/mL. Antibody validated: Western Blot in human, mouse and rat samples, Immunofluorescence in mouse samples, Immunohistochemistry in human, mouse, and rat samples, . All other applications and species not yet tested.

Images

Figure 2 Western Blot Validation in Mouse Liver Loading: 10 µg of lysates per lane.Antibodies: Albumin, 5159, 1 &956,g/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti- chicken IgY HRP conjugate at 1:10000 dilution.

Figure 3 Western Blot Validation in Rat Liver Loading: 10 µg of lysates per lane.Antibodies: Albumin, 5159, 1 &956,g/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti- chicken IgY HRP conjugate at 1:10000 dilution.

Figure 7 Immunofluorescence Validation of Albumin in Mouse Liver Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse liver tissue labeling albumin with 5159 at 20 /mL, followed by goat anti-chicken IgY secondary antibody at 1/500 dilution (red) and DAPI staining (blue).

Figure 4 Immunohistochemistry Validation of Albumin in Human Liver Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-albumin antibody (5159) at 2 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 &730,C. A goat anti- chicken IgY (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Figure 5 Immunohistochemistry Validation of Albumin in Mouse Liver Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-albumin antibody (5159) at 10 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 &730,C. A goat anti- chicken IgY (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Figure 6 Immunohistochemistry Validation of Albumin in Rat Liver Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-albumin antibody (5159) at 2 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 &730,C. A goat anti-chicken IgY (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Figure 1 Western Blot Validation in Human Liver Loading: 10 µg of lysates per lane.Antibodies: Albumin, 5159, 1 &956,g/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti- chicken IgY HRP conjugate at 1:10000 dilution.