SCF Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: SCF Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-5165
• Supplier Catalog Number: 5165
• Alternative Catalog Number: PRS-5165-0.02,PRS-5165-0.1
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Anti-SCF antibody (5165) was raised against a peptide corresponding to 18 amino acids near the center of human SCF. The immunogen is located within amino acids 100-150 of SCF.
• Conjugation: Unconjugated
• Alternative Names: SCF Antibody: SF, MGF, SCF, FPH2, KL-1, Kitl, SHEP7, Kit ligand, Mast cell growth factor

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/mL
• Molecular Weight: Predicted: 31kDObserved: 36 kD (31kD + 4 N-linked Glycosylations)
• NCBI: 4254
• UniProt: P21583
• Buffer: SCF Antibody is supplied in PBS containing 0.02% sodium azide.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1-2 µg/mL, IHC: 2.5 µg/mL, IF: 20 µg/mL.Antibody validated: Western Blot in human, mouse and rat samples, Immunohistochemistry in mouse samples, Immunofluorescence in human samples. All other applications and species not yet tested.

Images

Immunohistochemistry Validation of SCF in Mouse Brain Tissue Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-SCF antibody (5165) at 2.5 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 &730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Western Blot Validation with Recombinant ProteinLoading: 30 ng of human SCF recombinant protein per lane.Antibodies: SCF 5165 (Lane 1: 1 &956,g/mL and Lane 2: 2 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Observed at around 20kD.

Immunofluorescence Validation of SCF in Human Brain TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed human brain tissue labeling SCF with 5165 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Western Blot Validation in Cell Lines and Tissues of Human, Mouse and RatLoading: 15 &956,g of lysates per lane.Antibodies: SCF 5165 (1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Regulation Validation of SCF in Streptozotocin (STZ)-induced Diabetic Mice (Zhang et al., 2016) WB analysis showed protein expression level of SCF detected by anti-SCF antibody (5165) in gastric smooth muscle was significantly decreased in STZ-induced diabetic mice as compared to the control.

Regulated Expression Validation of SCF in Gastric Smooth Muscle Cells (GSMCs) of Mice (Zhang et al., 2016) WB analysis of protein expression level of SCF detected by anti-SCF antibody (5165). GSMCs were treated with PI3K inhibitor (LY294002), AT1R inhibitor (ZD7155) and AT2R inhibitor (PD123319) before Ang II treatment. Ang II (10-8mol/L) significantly increased SCF protein expression, which was reduced by treatment of LY294002 and ZD7155.

Regulated Expression Validation of SCF in GSMCs of Normal Mice (Wu et al., 2013) WB analysis of protein expression level of SCF detected by anti-SCF antibody (5165). GSMCs were treated with C-type natriuretic peptide (CNP) at different doses for 48hr. CNP(10-7mol/L and 10-6mol/L) significantly decreased SCF protein expression level as compared to the control group.