CD11b Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: CD11b Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-55-325
• Supplier Catalog Number: 55-325
• Alternative Catalog Number: PRS-55-325-400
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: FC, IHC-P, WB
• Species Reactivity: Human
• Immunogen: This CD11b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 253-282 amino acids from the N-terminal region of human CD11b.
• Conjugation: Unconjugated
• Alternative Names: Integrin alpha-M, CD11 antigen-like family member B, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor, CD11b, ITGAM, CD11B, CR3A

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: 127 kDa
• NCBI: 3684
• UniProt: P11215
• Buffer: Supplied in PBS with 0.09% (W/V) sodium azide.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: For IHC-P starting dilution is: 1:25For WB starting dilution is: 1:500For FACS starting dilution is: 1:10~50

Images

Antibody staining D11b in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

Antibody staining D11b in human spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

Antibody staining D11b in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

Western Blot at 1:2000 dilution + human spleen lysate Lysates/proteins at 20 ug per lane.

Western Blot at 1:500 dilution + human spleen lysate Lysates/proteins at 20 ug per lane.

Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis in A549,K562 cell line lysates (35ug/lane).