ZIP7 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: ZIP7 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-6093
• Supplier Catalog Number: 6093
• Alternative Catalog Number: PRS-6093-0.02,PRS-6093-0.1
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Anti-ZIP7 antibody (6093) was raised against a peptide corresponding to 17 amino acids near the amino terminus of human ZIP7. The immunogen is located within amino acids 20-70 of ZIP7.
• Conjugation: Unconjugated
• Alternative Names: ZIP7 Antibody: KE4, HKE4, ZIP7, RING5, H2-KE4, D6S115E, D6S2244E, Zinc transporter SLC39A7, Histidine-rich membrane protein Ke4

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/mL
• Molecular Weight: Predicted: 50kDObserved: 50kD
• NCBI: 7922
• UniProt: Q92504
• Buffer: ZIP7 Antibody is supplied in PBS containing 0.02% sodium azide.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 0.5-1 µg/mL, IHC: 2.5 µg/mL, IF: 20 µg/mL.Antibody validated: Western Blot in human, mouse and rat samples, Immunohistochemistry in human samples, Immunofluorescence in human samples. All other applications and species not yet tested.

Images

Figure 7 Immunofluorescence Validation of ZIP7 in Human Brain TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed human brain tissue labeling ZIP7 with 6093 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Figure 6 Immunohistochemistry Validation of ZIP7 in Human Brain Tissue Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-ZIP7 antibody (6093) at 2.5 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell LinesLoading: 15 &956,g of lysates per lane.Antibodies: ZIP7, 6093 (1 &956,g/mL), ZIP7, 24-029 (4 &956,g/mL), and beta-actin (1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Figure 2 Western Blot Validation in Human Cell LinesLoading: 15 &956,g of lysates per lane.Antibodies: ZIP7, 6093 (1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Figure 3 Western Blot Validation in Human Brain Tissue and 3T3 (Balb) Cell LysateLoading: 15 &956,g of lysates per lane.Antibodies: ZIP7, 6093 (1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Figure 4 Western Blot Validation in Mouse Brain Tissue LysateLoading: 15 &956,g of lysates per lane.Antibodies: ZIP7, 6093 (Lane 1: 0.5 &956,g/mL and Lane 2: 1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Figure 5 Western Blot Validation in Rat Brain Tissue LysateLoading: 15 &956,g of lysates per lane.Antibodies: ZIP7, 6093 (Lane 1: 0.5 &956,g/mL and Lane 2: 1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.