This VLDLR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 484-510 amino acids from the Central region of human VLDLR.
Conjugation:
Unconjugated
Alternative Names:
Very low-density lipoprotein receptor, VLDL receptor, VLDL-R, VLDLR
Optimal dilutions for each application to be determined by the researcher.
Application Notes:
For FACS starting dilution is: 1:25For IHC-P starting dilution is: 1:25For WB starting dilution is: 1:2000
Overlay histogram showing THP-1 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10 6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Antibody staining VLDLR in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Antibody staining VLDLR in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Western Blot at 1:2000 dilution + 293T/17 whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution + human heart lysate Lysates/proteins at 20 ug per lane.
VLDLR Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.
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