MMP12 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: MMP12 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-62-169
• Supplier Catalog Number: 62-169
• Alternative Catalog Number: PRS-62-169-400
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC-P, WB
• Species Reactivity: Human, Mouse
• Immunogen: This MMP12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 391-420 amino acids from the C-terminal region of human MMP12.
• Conjugation: Unconjugated
• Alternative Names: Macrophage metalloelastase, MME, Macrophage elastase, ME, hME, Matrix metalloproteinase-12, MMP-12, MMP12, HME

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: 54 kDa
• NCBI: 4321
• UniProt: P39900
• Buffer: Supplied in PBS with 0.09% (W/V) sodium azide.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: For IHC-P starting dilution is: 1:25For WB starting dilution is: 1:1000For IF starting dilution is: 1:10~50

Images

Antibody staining MMP12 in human lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

Western Blot at 1:2000 dilution + human heart lysate Lysates/proteins at 20 ug per lane.

Western Blot at 1:1000 dilution + human lung lysate Lysates/proteins at 20 ug per lane.

Western blot analysis of anti-hMMP12-R406 Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in 293 cell line lysate.

Western blot analysis of anti-hMMP12-R406 Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in mouse kidney tissue lysates.

Confocal immunofluorescent analysis of MMP12 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).