This PDIA6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 144-172 amino acids from the Central region of human PDIA6.
Conjugation:
Unconjugated
Alternative Names:
Protein disulfide-isomerase A6, Endoplasmic reticulum protein 5, ER protein 5, ERp5, Protein disulfide isomerase P5, Thioredoxin domain-containing protein 7, PDIA6, ERP5, P5, TXNDC7
Optimal dilutions for each application to be determined by the researcher.
Application Notes:
For IF starting dilution is: 1:100For IHC-P starting dilution is: 1:100For WB starting dilution is: 1:1000
Fluorescent image of HepG2 cells stained with XAF1 . Antibody was diluted at 1:100 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
Immunohistochemical analysis of paraffin-embedded H. liver section using . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Immunohistochemical analysis of paraffin-embedded H. brain section using . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Western blot analysis of lysates from K562, HepG2, HT-1080, rat C6 cell line and human liver tissue lysate (from left to right), using at 1:1000 at each lane.
Western blot analysis of in Y79 cell line lysates (35ug/lane)
Western blot analysis of in mouse stomach tissue lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human brain tissue reacted with , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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