This PCSK9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 144-173 amino acids from the N-terminal region of human PCSK9.
Optimal dilutions for each application to be determined by the researcher.
Application Notes:
For FACS starting dilution is: 1:25For IHC-P starting dilution is: 1:25For WB starting dilution is: 1:2000
Flow cytometric analysis of HeLa cells using PCSK9 Antibody (green) compared to an isotype control of rabbit IgG(blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Flow cytometric analysis of HeLa cells using PCSK9 Antibody (green) compared to an isotype control of rabbit IgG(blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Immunohistochemical analysis of paraffin-embedded H. colorectal carcinoma section using PCSK9 Antibody . Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Flow cytometric analysis of A431 cells using PCSK9 Antibody (green) compared to an isotype control of rabbit IgG(blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Western blot analysis of lysate from SW480 cell line, using PCSK9 Antibody at 1:2000.
Western blot analysis of lysates from HepG2, Hela, SW480 cell line (from left to right), using PCSK9 Antibody at 1:1000 at each lane.
Western blot analysis of lysate from Hela cell line, using PCSK9 Antibody at 1:1000.
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