Optimal dilutions for each application to be determined by the researcher.
Application Notes:
For FACS starting dilution is: 1:25For IHC-P starting dilution is: 1:25For WB starting dilution is: 1:1000
Overlay histogram showing A549 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10 6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Antibody staining ROR1 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Antibody staining ROR1 in Human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Immunohistochemical analysis of paraffin-embedded H. heart section using ROR1 Antibody . Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Western blot analysis of lysates from K562 cell line, human lung, mouse kidney, mouse heart tissue (from left to right), using ROR1 Antibody at 1:1000 at each lane.
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with the ROR1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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