This HLA-G antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-89 amino acids from the Central region of human HLA-G.
Conjugation:
Unconjugated
Alternative Names:
HLA class I histocompatibility antigen, alpha chain G, HLA G antigen, MHC class I antigen G, HLA-G, HLA-60, HLAG
Optimal dilutions for each application to be determined by the researcher.
Application Notes:
For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50
Western Blot at 1:2000 dilution Lane 1: Jurkat whole cell lysate Lane 2: Raji whole cell lysate Lane 3: Ramos whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of lysate from human placenta tissue lysate, using HLA-G Antibody ) at 1:1000 at each lane.
Western blot analysis of HLA-G Antibody in NCI-H460 cell line lysates (35ug/lane)
Western blot analysis of HLA-G using rabbit polyclonal HLA-G Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HLA-G gene.
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HLA-G Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometry analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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