SPT2 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: SPT2 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-6305
• Supplier Catalog Number: 6305
• Alternative Catalog Number: PRS-6305-0.02,PRS-6305-0.1
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, ICC, IF, WB
• Species Reactivity: Human, Mouse
• Immunogen: Anti-SPT2 antibody (6305) was raised against a peptide corresponding to 15 amino acids near the carboxy terminus of human SPT2. The immunogen is located within the last 50 amino acids of SPT2.
• Conjugation: Unconjugated
• Alternative Names: SPT2 Antibody: LCB2, SPT2, HSN1C, LCB2A, NSAN1C, hLCB2a, KIAA0526, LCB2, Serine palmitoyltransferase 2, Long chain base biosynthesis protein 2, LCB 2

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/mL
• Molecular Weight: Predicted: 63kDObserved: 63 kD
• NCBI: 9517
• UniProt: O15270
• Buffer: SPT2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 0.5-1 µg/mL, ICC: 10 µg/mL, IF: 20 µg/mL.Antibody validated: Western Blot in human and mouse samples, Immunocytochemistry in mouse samples, Immunofluorescence in mouse samples. All other applications and species not yet tested.

Images

Figure 3 Western Blot Validation in Mouse 3T3 Cell Lysate in (A) the absence and (B) the presence of blocking peptideLoading: 15 &956,g of lysates per lane.Antibodies: SPT2, 6305 (0.5 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Figure 6 Immunocytochemistry Validation of SPT2 in Mouse 3T3 CellsImmunocytochemical analysis of 3T3 cells using anti-SPT2 antibody (6305) at 10 &956,g/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Figure 7 Immunofluorescence Validation of SPT2 in Mouse 3T3 CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed 3T3 Cells labeling SPT2 with 6305 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Figure 1 Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell LinesLoading: 15 &956,g of lysates per lane.Antibodies: SPT2, 6305 (1 &956,g/mL), SPT2, 64-238 (1 &956,g/mL), and beta-actin (1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Figure 2 Western Blot Validation in Human Cell LinesLoading: 15 &956,g of lysates per lane.Antibodies: SPT2, 6305 (1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Figure 4 Western Blot Validation in Human Brain Tissue LysateLoading: 15 &956,g of lysates per lane.Antibodies: SPT2, 6305 (Lane 1: 0.5 &956,g/mL and Lane 2: 1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Figure 5 Western Blot Validation in Mouse 3T3/NIH Cell LysateLoading: 15 &956,g of lysates per lane.Antibodies: SPT2, 6305 (Lane 1: 0.5 &956,g/mL and Lane 2: 1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.