This GLG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1152-1179 amino acids from the C-terminal region of human GLG1.
Optimal dilutions for each application to be determined by the researcher.
Application Notes:
For WB starting dilution is: 1:2000For IHC-P starting dilution is: 1:50~100
Western Blot at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: T47D whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: MCF-7 lysate Lane 2: HepG2 whole cell lysate Lane 3: T47D whole cell lysate Lane 4: mouse cerebellum lysate Lane 5: human placenta lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: mouse cerebellum lysate Lane 3: MCF-7 whole cell lysate Lane 4: T47D whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in HepG2,T47D cell line lysates (35ug/lane).
Western blot analysis of GLG1 Antibody in mouse cerebellum tissue lysates (35ug/lane)
GLG1 Antibody IHC analysis in formalin fixed and paraffin embedded skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.
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