JMJD3 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: JMJD3 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-6657
• Supplier Catalog Number: 6657
• Alternative Catalog Number: PRS-6657-0.02,PRS-6657-0.1
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, ICC, IF, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Anti-JMJD3 antibody (6657) was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human JMJD3. The immunogen is located within amino acids 1370-1420 of JMJD3.
• Conjugation: Unconjugated
• Alternative Names: JMJD3 Antibody: JMJD3, JMJD3, KIAA0346, Lysine-specific demethylase 6B, JmjC domain-containing protein 3

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/mL
• Molecular Weight: Predicted: 180Observed: 180 kD
• NCBI: 23135
• UniProt: O15054
• Buffer: JMJD3 Antibody is supplied in PBS containing 0.02% sodium azide.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 0.25-4 µg/mL, ICC: 2.5 µg/mL, IF: 20 µg/mL.Antibody validated: Western Blot in human, mouse and rat samples, Immunocytochemistry in human samples, Immunofluorescence in human samples. All other applications and species not yet tested.

Images

Figure 3 Western Blot Validation in HeLa Cell LysateLoading: 15 &956,g of lysates per lane.Antibodies: JMJD3, 6657 (0.25 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Figure 7 Immunocytochemistry Validation of JMJD3 in K562 CellsImmunocytochemical analysis of K562 cells using anti-JMJD3 antibody (6657) at 2.5 &956,g/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Figure 8 Immunofluorescence Validation of JMJD3 in K562 CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling JMJD3 with 6657 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Figure 1 Western Blot Validation in Human, Mouse and Rat Cell LinesLoading: 15 &956,g of lysates per lane.Antibodies: JMJD3, 6657, (1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines Loading: 15 &956,g of lysates per lane.Antibodies: JMJD3 6657 (1 &956,g/mL), JMJD3 14-618, (2 &956,g/mL) and beta-actin 3779 (1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Figure 4 Western Blot Validation in K562 Cell Lysate Loading: 15 &956,g of lysates per lane.Antibodies: : JMJD3, 6657 (0.5 &956,g/mL) , 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: in the absence and Lane 2: in the presence of blocking peptide

Figure 5 Western Blot Validation in Mouse A-20 Cell Lysate Loading: 15 &956,g of lysates per lane.Antibodies: : JMJD3, 6657 (0.5 &956,g/mL) , 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: in the absence and Lane 2: in the presence of blocking peptide

Figure 6 Western Blot Validation in HepG2 Cell Lysate Loading: 15 &956,g of lysates per lane.Antibodies: : JMJD3, 6657, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: 0.25 &956,g/mLLane 2: 0.5 &956,g/mL Lane 3: 0.25 &956,g/mL with blocking peptide