LMX1A Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: LMX1A Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-7087
• Supplier Catalog Number: 7087
• Alternative Catalog Number: PRS-7087-0.02,PRS-7087-0.1
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, ICC, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Rabbit polyclonal LMX1A antibody was raised against a 16 amino acid peptide near the carboxy terminus of human LMX1A.The immunogen is located within amino acids 300 - 350 of LMX1A.
• Conjugation: Unconjugated
• Alternative Names: LMX1A Antibody: LMX1, LMX1.1, LIM homeobox transcription factor 1-alpha

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/mL
• Molecular Weight: Predicted: 42kD Observed: 45 kD
• NCBI: 4009
• UniProt: Q8TE12
• Buffer: LMX1A Antibody is supplied in PBS containing 0.02% sodium azide.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1-2 µg/mL, ICC/IF: 10-20 µg/mL, IHC-P: 1-5 µg/mL. Antibody validated: Western Blot in human, mouse and rat samples, Immunocytochemistry in human samples, Immunohistochemistry in human, mouse, and rat samples. All other applications and species not yet tested.

Images

Figure 2 Western Blot Validation in Human Skeletal Muscle Loading: 10 &956,g of lysates per lane.A&956,g ntibodies: LMX1A, 7087, 1 &956,g/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Figure 3 Western Blot Validation in Mouse Skeletal Muscle Loading: 10 &956,g of lysates per lane.Antibodies: LMX1A, 7087, 1 &956,g/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Figure 1 Immunofluorescence Validation of LMX1A in HeLa CellsImmunofluorescent analysis of methanol-fixed HeLa cells labeling LMX1A with 7087 at 10 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and DAPI staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.

Figure 5 Immunohistochemistry Validation of LMX1A in Human Brain Meningioma Immunohistochemical analysis of paraffin-embedded human brain meningioma using anti-LMX1A antibody (7087) at 2 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Figure 6 Immunohistochemistry Validation of LMX1A in Mouse Brain Tissue Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti- anti-LMX1A antibody (7087) at 2 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Figure 7 Immunohistochemistry Validation of LMX1A in Rat Skeletal Muscle Tissue Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-LMX1A antibody (7087) at 5 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Figure 4 Western Blot Validation in Rat Skeletal Muscle Loading: 10 &956,g of lysates per lane.Antibodies: LMX1A, 7087, 1 &956,g/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.