CRBN Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: CRBN Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-8115
• Supplier Catalog Number: 8115
• Alternative Catalog Number: PRS-8115-0.02,PRS-8115-0.1
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: CRBN antibody was raised against a 16 amino acid peptide near the carboxy terminus of human CRBN.The immunogen is located within the last 50 amino acids of CRBN.
• Conjugation: Unconjugated
• Alternative Names: Cereblon, AD-006, DKFZp781K0715, MGC27358, MRT2A

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/mL
• Molecular Weight: Predicted: 49 kDa Observed: 54 kDa
• NCBI: 51185
• UniProt: Q96SW2
• Buffer: CRBN antibody is supplied in PBS containing 0.02% sodium azide.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: CRBN antibody can be used for detection of CRBN by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.Antibody validated: Western Blot in human samples, Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.

Images

Figure 2 WB Validation in Human Testis Loading: 15 &956,g of human testis lysate Antibodies: CRBN 8115, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane A: 0.5 &956,g/mLLane B: 1 &956,g/mL

Figure 3 WB Validation in Human Cells Loading: 15 &956,g of cell lysate Antibodies: CRBN 8115, 1 &956,g/mL,1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Figure 1 Immunofluorescence Validation of CRBN in HeLa CellsImmunofluorescent analysis of methanol-fixed HeLa cells labeling CRBN with 8115 at 10 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and Hoechst staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.

Figure 4 WB Validation in Mouse Tissues Loading: 15 &956,g of mouse tissue lysate Antibodies: CRBN 8115, 0.5 &956,g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Figure 5 WB Validation in Rat Tissues Loading: 15 &956,g of rat tissue lysate Antibodies: CRBN 8115, 0.5 &956,g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.