MAGEA4 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: MAGEA4 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-8191
• Supplier Catalog Number: 8191
• Alternative Catalog Number: PRS-8191-0.02,PRS-8191-0.1
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: MAGEA4 antibody was raised against a 16 amino acid peptide near the amino terminus of human MAGEA4.The immunogen is located within the first 50 amino acids of MAGEA4.
• Conjugation: Unconjugated
• Alternative Names: Melanoma antigen family A4, MAGE4, MAGE-41, MAGE4A, MAGE-4 antigen, MAGE4B, MAGE-X2, MAGE-X2 antigen, Melanoma-associated antigen 4

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/mL
• Molecular Weight: Predicted: 35 kDa Observed: 45 kDa
• NCBI: 4103
• UniProt: P43358
• Buffer: MAGEA4 antibody is supplied in PBS containing 0.02% sodium azide.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: MAGEA4 antibody can be used for detection of MAGEA4 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL. Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Images

Western blot analysis of MAGEA4 in A431 cell lysate with MAGEA4 antibody at 1 &956,g/ml.

Immunocytochemistry of MAGEA4 in HeLa cells with MAGEA4 antibody at 5 &956,g/ml.

Immunofluorescence of MAGEA4 in human breast cancer tissue with MAGEA4 antibody at 20 &956,g/ml.

Immunofluorescence of MAGEA4 in human breast cancer tissue with MAGEA4 antibody at 20 &956,g/ml.Green: MAGEA4 Antibody (8191) Blue: DAPI staining

Immunofluorescence of MAGEA4 inHeLa cells with MAGEA4 antibody at 20 &956,g/ml.Red: MAGEA4 Antibody (8191) Blue: DAPI staining

Immunohistochemistry of MAGEA4 in human breast tumor with MAGEA4 antibody at 5 &956,g/mL.