hRIP3 Antibody, Unconjugated, Rabbit, Polyclonal

Article Name:	hRIP3 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number:	PRS-8963
Supplier Catalog Number:	8963
Alternative Catalog Number:	PRS-8963-0.02,PRS-8963-0.1
Manufacturer:	ProSci
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, IF, IHC-P, WB
Species Reactivity:	Human
Immunogen:	Anti-hRIP3 antibody (8963) was raised against a peptide corresponding to 18 amino acids near the carboxy terminus of human hRIP3. The immunogen is located within the last 50 amino acids of hRIP3.
Conjugation:	Unconjugated
Alternative Names:	hRIP3 Antibody: Rip3, AW107945, 2610528K09Rik, RIP-like protein kinase 3, RIP-3

Clonality:	Polyclonal
Concentration:	1 mg/ml
Molecular Weight:	Predicted: 57kDObserved: 57 kD
NCBI:	11035
UniProt:	Q9Y572
Buffer:	hRIP3 Antibody is supplied in PBS containing 0.02% sodium azide.
Form:	Liquid

Application Dilute:	Optimal dilutions for each application to be determined by the researcher.
Application Notes:	WB: 0.5-1 µg/mL, IF: 20 µg/mL, IHC: 2-5 µg/mL.Antibody validated: Western Blot, Immunofluorescence and Immunohistochemistry in human samples. All other applications and species not yet tested.

	Figure 4 Immunofluorescence Validation of hRIP3 in Molt4 CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed Molt4 cells labeling hRIP3 with 8963 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
	Figure 5 Immunofluorescence Validation of hRIP3 in Human Breast TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed human breast tissue labeling hRIP3 with 8963 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
	Figure 6 Immunofluorescence Validation of hRIP3 in Human Colon TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed human colon tissue labeling hRIP3 with 8963 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
	Figure 7 Immunohistochemistry Validation of hRIP3 in Human Liver Tissue Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-hRIP3 antibody (8963) at 5 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
	Figure 8 Immunohistochemistry Validation of hRIP3 in Human Breast Tissue Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-hRIP3 antibody (8963) at 5 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
	Figure 9 Immunohistochemistry Validation of hRIP3 in Human Colon Tissue Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-hRIP3 antibody (8963) at 2 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
	Figure 1 Western Blot Validation in Human Cell LinesLoading: 15 &956,g of lysates per lane.Antibodies: hRIP3, 8963 (0.5 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 2 Western Blot Validation in Human TissuesLoading: 15 &956,g of lysates per lane.Antibodies: hRIP3, 8963 (1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 3 Western Blot Validation of hRIP3 in Human THP-1 Cell LysateLoading: 15 &956,g of lysates per lane.Antibodies: hRIP3, 8963 (1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1-2: human THP-1 cell lysate in the absence (Lane 1) or the presence (Lane 2) of blocking peptide.