SARS-CoV-2 (COVID-19) Spike S1 Antibody (biotin), Biotin, Rabbit, Polyclonal

Product Overview

• Article Name: SARS-CoV-2 (COVID-19) Spike S1 Antibody (biotin), Biotin, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-9083-BIOTIN
• Supplier Catalog Number: 9083-biotin
• Alternative Catalog Number: PRS-9083-BIOTIN-0.02,PRS-9083-BIOTIN-0.1
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: ELISA
• Species Reactivity: Virus
• Immunogen: Anti-SARS-CoV-2 (COVID-19) Spike S1 Antibody (biotin) (9083-biotin) was raised against a peptide corresponding to 16 amino acids near the amino terminus of SARS-CoV-2 (COVID-19) Spike S1 glycoprotein. The immunogen is located within the first 50 amino acids of SARS-CoV-2 (COVID-19) Spike S1 protein.
• Conjugation: Biotin
• Alternative Names: SARS-CoV-2 (COVID-19) Spike S1 Antibody (biotin): Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Spike protein, Surface Glycoprotein, covid-19, sars-cov-2

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/mL
• NCBI: 43740568
• UniProt: P0DTC2
• Buffer: SARS-CoV-2 (COVID-19) Spike S1 Antibody (biotin) is supplied in PBS containing 0.02% sodium azide.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: SARS-CoV-2 Spike S1 antibody can be used for as detection antibody in Sandwich Elisa at 1 µg/mL.

Images

Figure 1 ELISA Validation of SARS-CoV-2 Spike Antibody PairA sandwich ELISA was performed using the anti-SARS-COV-2 Spike S1 antibodies 9091 (10 &956,g/mL ) as the capture antibody. Biotin-labled anti-SARS-COV-2 Spike S1 antibodies 9083-biotin (1 &956,g/mL ) and streptavidin-HRP (0.1 &956,g/mL ) were used for detection. Detection range is from 0.8 ng to 200 ng (SARS-CoV-2 Spike S1 Protein, 97-087).

Figure 2 Sandwich ELISA for SARS-CoV-2 (COVID-19) Matched Pair Spike S1 AntibodiesAntibodies: SARS-CoV-2 (COVID-19) Spike Antibodies, 10-350 and 9083-biotin. A sandwich ELISA was performed using SARS-CoV-2 Spike S1 antibody (10-350, 2ug/ml) as capture antibody, the Spike S1 recombinant protein as the binding protein (97-087), and the anti-SARS-CoV-2 Spike S1 antibody (9083-biotin, 1ug/ml) as the detection antibody. Secondary: Streptavidin-HRP at 1:10000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 5.1 ng

Figure 3 Sandwich ELISA for SARS-CoV-2 (COVID-19) Matched Pair Spike S1 AntibodiesAntibodies: SARS-CoV-2 (COVID-19) Spike Antibodies, 10-351 and 9083-biotin. A sandwich ELISA was performed using SARS-CoV-2 Spike S1 antibody (10-351, 2ug/ml) as capture antibody, the Spike S1 recombinant protein as the binding protein (97-087), and the anti-SARS-CoV-2 Spike S1 antibody (9083-biotin, 1ug/ml) as the detection antibody. Secondary: Streptavidin-HRP at 1:10000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 1.96 ng