Anti-SARS-CoV-2 (COVID-19) Nucleocapsid Antibody (biotin) (9099-biotin) was raised against a peptide corresponding to 17 amino acids near the center of SARS-CoV-2 (COVID-19) Nucleocapsid protein. The immunogen is located within 230-280 amino acids of SARS-CoV-2 (COVID-19) Nucleocapsid protein.
Conjugation:
Biotin
Alternative Names:
N protein, Nucleocapsid Phosphoprotein, Nucleocapsid protein, SARS-CoV-2 (COVID-19) Nucleocapsid Antibody (biotin): Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), covid-19, sars-cov-2
SARS-CoV-2 (COVID-19) Nucleocapsid Antibody (biotin) is supplied in PBS containing 0.02% sodium azide.
Form:
Liquid
Application Dilute:
Optimal dilutions for each application to be determined by the researcher.
Application Notes:
IHC: 1 µg/mL Antibody validated: Immunohistochemistry in COVID-19 patient samples. All other applications and species not yet tested.
Figure 2 ELISA Validation Coating Antigen: immunogen peptide, 9099P, 10 &956,g/mL, incubate at 4 &730,C overnight.Detection Antibodies: SARS-CoV-2 Spike antibody, 9099-biotin or 9099, dilution: 0.5-1000 ng/mL, incubate at RT for 1 hr. 9099-biotin was detected by HRP-conjugated streptavidin at 1:5,000 and 9099 was detected by anti-rabbit HRP conjugated secondary antibodies at 1:10,000 , incubate at RT for 1 hr.
Figure 1 Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) Nucleocapsid Protein in COVID-19 Patient Lung Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) nucleocapsid antibody (9099-HRP, 2 &956,g/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C, following by streptavidin-HRP conjugate at 20 &956,g/mL. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 nucleocapsid protein was observed in macrophages of COVID-19 patient lung, but not in non-COVID-19 patient lung.
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