SARS-CoV-2 (COVID-19) Nucleocapsid Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: SARS-CoV-2 (COVID-19) Nucleocapsid Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-9103
• Supplier Catalog Number: 9103
• Alternative Catalog Number: PRS-9103-0.02,PRS-9103-0.1
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Virus
• Immunogen: Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody (9103) was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of SARS-CoV-2 (COVID-19) Nucleocapsid protein. The immunogen is located within 350-400 amino acids of SARS-CoV-2 (COVID-19) Nucleocapsid protein.
• Conjugation: Unconjugated
• Alternative Names: N protein, Nucleocapsid Phosphoprotein, Nucleocapsid protein, SARS-CoV-2 (COVID-19) Nucleocapsid Antibody: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), covid-19, sars-cov-2

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/mL
• NCBI: 43740575
• Buffer: SARS-CoV-2 (COVID-19) Nucleocapsid Antibody is supplied in PBS containing 0.02% sodium azide.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 0.5-2 µg/mLSARS-CoV-2 (COVID-19) Nucleocapsid antibody can be used for the detection of SARS-CoV-2 (COVID-19) Nucleocapsid protein in ELISA. It will detect 4 ng of free peptide at 1 µg/mL. Antibody can also be used for as capture antibody in Sandwich Elisa at 10 µg/mL. All other applications and species not yet tested.

Images

Figure 3 ELISA Validation with SARS-CoV-2 (COVID-19) Nucleocapsid Recombinant ProteinAntibodies: SARS-CoV-2 (COVID-19) Nucleocapsid antibody, 9103 (1 &956,g/mL). A direct ELISA was performed using SARS-CoV-2 (COVID-19) Nucleocapsid recombinant protein as coating antigen and the anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000ng/mL.

Figure 4 ELISA Validation of SARS-CoV-2 Nucleocapsid Antibody PairA sandwich ELISA was performed using the anti-SARS-COV-2 Nucleocapsid antibodies 9103 (10 &956,g/mL ) as the capture antibody. Biotin-labled anti-SARS-COV-2 Nucleocapsid antibodies 9099-biotin (1 &956,g/mL ) and streptavidin-HRP (0.1 &956,g/mL ) were used for detection. Detection range is from 3 ng to 200 ng (SARS-CoV-2 Nucleocapsid Protein, 10-306).

Figure 1 Western Blot Validation with SARS-CoV-2 (COVID-19) Nucleocapsid Recombinant Protein Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) Nucleocapsid recombinant protein, 10-306. Antibodies: SARS-CoV-2 (COVID-19) Nucleocapsid, 9103, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 &956,g/mL and Lane 2: 2 &956,g/mL.

Figure 2 Western Blot Validation with SARS-CoV-2 (COVID-19) Nucleocapsid Recombinant ProteinLoading: 50 ng per lane of SARS-CoV-2 (COVID-19) Nucleocapsid recombinant protein. Antibodies: SARS-CoV-2 (COVID-19) Nucleocapsid, 9103, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.5 &956,g/mL, Lane 2: 1 &956,g/mL and Lane 3: 2 &956,g/mL

Figure 5 Sandwich ELISA for SARS-CoV-2 (COVID-19) Matched Pair Nucleocapsid AntibodiesAntibodies: SARS-CoV-2 (COVID-19) Nucleocapsid Antibodies, 35-720 and 9103. A sandwich ELISA was performed using SARS-CoV-2 Nucleocapsid antibody (35-720, 5ug/ml) as capture antibody, the Nucleocapsid recombinant protein as the binding protein (10-306), and the anti-SARS-CoV-2 Nucleocapsid antibody (9103, 1ug/ml) as the detection antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 6.9 ng