SARS-CoV-2 (COVID-19) Spike S2 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: SARS-CoV-2 (COVID-19) Spike S2 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-9119
• Supplier Catalog Number: 9119
• Alternative Catalog Number: PRS-9119-0.02,PRS-9119-0.1
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Virus
• Immunogen: Anti-SARS-CoV-2 (COVID-19) Spike antibody (9119) was raised against a peptide corresponding to 16 amino acids near the center terminus of SARS-CoV-2 (COVID-19) Spike glycoprotein. The immunogen is located within 900-950 amino acids of SARS-CoV-2 (COVID-19) Spike protein.
• Conjugation: Unconjugated
• Alternative Names: SARS-CoV-2 (COVID-19) Spike Antibody: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Spike protein, Surface Glycoprotein, covid-19, sars-cov-2

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/mL
• NCBI: 43740568
• UniProt: P0DTC2
• Buffer: SARS-CoV-2 (COVID-19) Spike Antibody is supplied in PBS containing 0.02% sodium azide.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1 µg/mL.Antibody validated: SARS-CoV-2 (COVID-19) Spike antibody can be used for the detection of SARS-CoV-2 (COVID-19) Spike protein in WB. It will detect 2 ng of free peptide at 1 µg/mL. All other applications and species not yet tested.

Images

Figure 2 ELISA Validation Antibodies: SARS-CoV-2 (COVID-19) Spike S2 Antibody, 9181. A direct ELISA was performed using immunogen peptide (9119P) as coating antigen and the anti-SARS-CoV-2 (COVID-19) Spike S2 antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL.

Figure 1 Western Blot Validation with SARS-CoV-2 (COVID-19) Spike S2 ECD Recombinant ProteinLoading: 30 ng per lane of SARS-CoV-2 (COVID-19) Spike S2 ECD recombinant protein, 10-115. Antibodies: SARS-CoV-2 (COVID-19) Spike S2, 9119, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 &956,g/mL and Lane 2: 2 &956,g/mL.