SARS-CoV-2 (COVID-19) Spike S2 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: SARS-CoV-2 (COVID-19) Spike S2 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-9123
• Supplier Catalog Number: 9123
• Alternative Catalog Number: PRS-9123-0.02,PRS-9123-0.1
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Virus
• Immunogen: Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody (9123) was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of SARS-CoV-2 (COVID-19) Spike glycoprotein. The immunogen is located within 1130-1180 amino acids of SARS-CoV-2 (COVID-19) Spike protein.
• Conjugation: Unconjugated
• Alternative Names: SARS-CoV-2 (COVID-19) Spike Antibody: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Spike protein, Surface Glycoprotein, covid-19, sars-cov-2

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/mL
• NCBI: 43740568
• UniProt: P0DTC2
• Buffer: SARS-CoV-2 (COVID-19) Spike Antibody is supplied in PBS containing 0.02% sodium azide.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1 µg/mL, IHC: 0.1 µg/mL, IF: 10 µg/mL.Antibody validated: Immunohistochemistry and Immunofluorescence in human samples. SARS-CoV-2 (COVID-19) Spike antibody can be used for the detection of SARS-CoV-2 (COVID-19) Spike protein in ELISA. It will detect 4 ng of free peptide at 1 µg/mL. All other applications and species not yet tested.

Images

Figure 2 ELISA Validation with SARS-CoV-2 (COVID-19) Spike S2 ECD Recombinant ProteinAntibodies: SARS-CoV-2 (COVID-19) Spike S2 antibody, 9123. A direct ELISA was performed using SARS-CoV-2 (COVID-19) Spike S2 ECD recombinant protein (10-115) as coating antigen and the anti-SARS-CoV-2 (COVID-19) Spike S2 antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 8 ng/mL to 1000ng/mL.

Figure 1 Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) Spike S2 in COVID-19 Patient Lung Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) Spike S2 antibody (9123, 0.1 &956,g/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 spike protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.

Figure 3 Western Blot Validation with SARS-CoV-2 (COVID-19) Spike S2 ECD Recombinant ProteinLoading: 30 ng per lane of SARS-CoV-2 (COVID-19) Spike S2 ECD recombinant protein, 10-115. Antibodies: SARS-CoV-2 (COVID-19) Spike S2, 9123, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 &956,g/mL and Lane 2: 2 &956,g/mL.

Figure 4 Overexpression Validation in Spike Transfected 293 Cells Loading: 10 &956,g per lane of Spike transfected 293 cell lysate. Antibodies: SARS-CoV-2 (COVID-19) Spike, 9123, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 &956,g/mL and Lane 2: 2 &956,g/mL