SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-9151
• Supplier Catalog Number: 9151
• Alternative Catalog Number: PRS-9151-0.02,PRS-9151-0.1
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Virus
• Immunogen: Anti-SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) antibody (9151) was raised against a peptide corresponding to 15 amino acids in the center of SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) protein. The immunogen is located within 80-130 amino acids of the SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) protein.
• Conjugation: Unconjugated
• Alternative Names: 3C-like proteinase, 3CLp, Main protease, Mpro, Non-structure protein 5, covid-19, nsp5, sars-cov-2

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/mL
• NCBI: 43740578
• UniProt: P0DTC1
• Buffer: SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) Antibody is supplied in PBS containing 0.02% sodium azide.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 0.5 µg/mLAntibody validated: SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) antibody can detect 2 ng of free peptide at 1 µg/mL in ELISA.

Images

Figure 2 Western Blot Validation with SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) ProteinLoading: 30 ng per lane of SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) recombinant protein (10-400).Antibodies: SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5), 9151, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: 0.5 &956,g/mL and Lane 2: 1 &956,g/mL

Figure 1 ELISA Validation with SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) Protein Antibodies: SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) Antibody, 9151. A direct ELISA was performed using SARS-CoV-2 3CL-PRO (NSP5) recombinant protein (10-400) as coating antigen and the anti-SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/mL