SARS-CoV-2 (COVID-19) Membrane Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: SARS-CoV-2 (COVID-19) Membrane Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-9157
• Supplier Catalog Number: 9157
• Alternative Catalog Number: PRS-9157-0.02,PRS-9157-0.1
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IHC, WB
• Species Reactivity: Virus
• Immunogen: Anti-SARS-CoV-2 (COVID-19) Membrane antibody (9157) was raised against a peptide corresponding to 13 amino acids near the center of SARS-CoV-2 (COVID-19) Membrane protein. The immunogen is located between 130-180 amino acids of the SARS-CoV-2 (COVID-19) Membrane protein.
• Conjugation: Unconjugated
• Alternative Names: E1 glycoprotein, M protein, Membrane protein, SARS Matrix protein, SARS-CoV-2/SARS-CoV Matrix Antibody: SARS-CoV-2 Matrix protein, covid-19, sars-cov-2

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/mL
• NCBI: 43740571
• Buffer: SARS-CoV-2 (COVID-19) Membrane Antibody is supplied in PBS containing 0.02% sodium azide.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 0.25 µg/mL, IHC: 0.5 µg/mLAntibody validated: Immunohistochemistry in human samples. SARS-CoV-2 (COVID-19) Membrane antibody can detect 2 ng of free peptide at 1 µg/mL in ELISA. It can detect SARS-CoV-2 Membrane recombinant protein by ELISA and WB. All other applications and species not yet tested.

Images

Figure 2 ELISA Validation with SARS-CoV-2 (COVID-19) Membrane Protein Antibodies: SARS-CoV-2 (COVID-19) Membrane Antibody, 9157. A direct ELISA was performed using SARS-CoV-2 membrane recombinant protein (10-429) as coating antigen and the anti-SARS-CoV-2 (COVID-19) Membrane antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/ml

Figure 1 Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) Membrane in COVID-19 Patient Lung Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) Membrane antibody (9157, 0.5 &956,g/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 Membrane protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.

Figure 3 Western Blot Validation with SARS-CoV-2 (COVID-19) Membrane ProteinLoading: 30 ng per lane of SARS-CoV-2 (COVID-19) membrane protein (10-429).Antibodies: SARS-CoV-2 (COVID-19) membrane, 9157, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: 0.25 &956,g/mL and Lane 2: 0.5 &956,g/mL