SARS-CoV-2 (COVID-19) NSP13 (Helicase) Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: SARS-CoV-2 (COVID-19) NSP13 (Helicase) Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-9183
• Supplier Catalog Number: 9183
• Alternative Catalog Number: PRS-9183-0.02,PRS-9183-0.1
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IHC
• Species Reactivity: Virus
• Immunogen: Anti-SARS-CoV-2 (COVID-19) NSP13 (Helicase)antibody (9183) was raised against a peptide corresponding to 14 amino acids near the center of the SARS-CoV-2 (COVID-19) NSP13 (Helicase) protein. The immunogen is located between 440-490 amino acids of SARS-CoV-2 (COVID-19) NSP13 (Helicase).
• Conjugation: Unconjugated
• Alternative Names: Helicase, NSP13, Non-structural protein 13, covid-19, sars-cov-2

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/mL
• NCBI: 43740578
• UniProt: P0DTC1
• Buffer: SARS-CoV-2 (COVID-19) NSP13 (Helicase) Antibody is supplied in PBS containing 0.02% sodium azide.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: IHC: 0.5 µg/mL.Antibody validated: Immunohistochemistry in human samples. SARS-CoV-2 (COVID-19) NSP13 antibody can detect 2 ng of free peptide at 1 µg/mL in ELISA. It can detect SARS-CoV-2 NSP13 recombinant protein by ELISA. All other applications and species not yet tested

Images

Figure 3 ELISA Validation Antibodies: SARS-CoV-2 (COVID-19) NSP13 (Helicase) Antibody, 9183. A direct ELISA was performed using immunogen peptide (9183P) as coating antigen and the anti-SARS-CoV-2 (COVID-19) NSP13 (Helicase) antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL

Figure 1 Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) NSP13 in COVID-19 Patient Lung Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) NSP13 antibody (9183, 0.5 &956,g/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 NSP13 protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.