SARS-CoV-2 (COVID-19) NSP16 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: SARS-CoV-2 (COVID-19) NSP16 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-9271
• Supplier Catalog Number: 9271
• Alternative Catalog Number: PRS-9271-0.02,PRS-9271-0.1
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IHC, WB
• Species Reactivity: Virus
• Immunogen: Anti-SARS-CoV-2 (COVID-19) NSP16 antibody (9271) was raised against a peptide corresponding to 16 amino acids near the central domain of SARS-CoV-2 (COVID-19) NSP16 protein. The immunogen is located within 120-170 amino acids of the SARS-CoV-2 (COVID-19) NSP16 protein.
• Conjugation: Unconjugated
• Alternative Names: 2-O-methyltransferase, Non-structure protein 16, covid-19, nsp16, sars-cov-2

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/mL
• NCBI: 43740578
• UniProt: P0DTC1
• Buffer: SARS-CoV-2 (COVID-19) NSP16 antibody is supplied in PBS containing 0.02% sodium azide.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: IHC: 0.5 µg/mL, WB: 0.5 µg/mLAntibody validated: Immunohistochemistry in human samples. SARS-CoV-2 (COVID-19) NSP16 antibody can detect 2 ng of free peptide at 1 µg/mL in ELISA. It can detect SARS-CoV-2 NSP16 recombinant protein by ELISA and WB. All other applications and species not yet tested.

Images

Figure 2 ELISA Validation with SARS-CoV-2 (COVID-19) NSP16 Protein Antibodies: SARS-CoV-2 (COVID-19) NSP16 Antibody, 9271. A direct ELISA was performed using SARS-CoV-2 NSP16 recombinant protein (10-418) as coating antigen and the anti-SARS-CoV-2 (COVID-19) NSP16 antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/mL

Figure 1 Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) NSP16 in COVID-19 Patient Lung Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) NSP16 antibody (9271, 0.5 &956,g/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 NSP16 protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.

Figure 3 Western Blot Validation with SARS-CoV-2 (COVID-19) NSP16 ProteinLoading: 30 ng per lane of SARS-CoV-2 (COVID-19) NSP16 recombinant protein (10-418).Antibodies: SARS-CoV-2 (COVID-19) NSP16, 9271, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: 1 &956,g/mL and Lane 2: 2 &956,g/mL