SARS-CoV-2 (COVID-19) ORF3a Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: SARS-CoV-2 (COVID-19) ORF3a Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-9275
• Supplier Catalog Number: 9275
• Alternative Catalog Number: PRS-9275-0.02,PRS-9275-0.1
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IHC
• Species Reactivity: Virus
• Immunogen: Anti-SARS-CoV-2 (COVID-19) ORF3a antibody (9275) was raised against a peptide corresponding to 15 amino acids near the amino-terminus of SARS-CoV-2 (COVID-19) ORF3a protein. The immunogen is located within the first 50 amino acids of the SARS-CoV-2 (COVID-19) ORF3a protein.
• Conjugation: Unconjugated
• Alternative Names: Accessory protein 3a, ORF3a, ORF3a protein, Protein 3a, Protein U274, Protein X1, covid-19, sars-cov-2

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/mL
• NCBI: 43740569
• UniProt: P0DTC3
• Buffer: SARS-CoV-2 (COVID-19) ORF3a antibody is supplied in PBS containing 0.02% sodium azide.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: IHC: 0.2 µg/mLAntibody validated: Immunohistochemistry in human samples. Antibody validated: SARS-CoV-2 (COVID-19) ORF3a antibody can detect 2 ng of free peptide at 1 µg/mL in ELISA. All other applications and species not yet tested.

Images

Figure 2 ELISA Validation Antibodies: SARS-CoV-2 (COVID-19) ORF3a Antibody, 9275. A direct ELISA was performed using SARS-CoV-2 ORF3a immunogen peptide (9275P)) as coating antigen and the anti-SARS-CoV-2 (COVID-19) ORF3a antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL

Figure 1 Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) ORF3a in COVID-19 Patient Lung Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) ORF3a antibody (9275, 0.2 &956,g/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 ORF3a protein was observed in macrophages of COVID-19 patient lung, but not in non-COVID-19 patient lung.