SARS-CoV-2 (COVID-19) ORF3b Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: SARS-CoV-2 (COVID-19) ORF3b Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-9277
• Supplier Catalog Number: 9277
• Alternative Catalog Number: PRS-9277-0.02,PRS-9277-0.1
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Virus
• Immunogen: Anti-SARS-CoV-2 (COVID-19) ORF3b antibody (9277) was raised against a peptide corresponding to 12 amino acids near carboxyl terminus of SARS-CoV-2 (COVID-19) ORF3b protein. The immunogen is located within the last 50 amino acids of the SARS-CoV-2 (COVID-19) ORF3b protein.
• Conjugation: Unconjugated
• Alternative Names: Acessary protein 3b, Non-structural protein 3b, ORF3b, ORF3b protein, covid-19, ns3b, sars-cov-2

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/mL
• Molecular Weight: 17kD
• NCBI: 43740569
• UniProt: P0DTC4
• Buffer: SARS-CoV-2 (COVID-19) ORF3b antibody is supplied in PBS containing 0.02% sodium azide.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 0.1 µg/mLAntibody validated: SARS-CoV-2 (COVID-19) ORF3b antibody can detect 2 ng of free peptide at 1 µg/mL in ELISA. It can detect SARS-CoV-2 ORF3b recombinant protein by ELISA and WB. All other applications and species not yet tested.

Images

Figure 1 ELISA Validation with SARS-CoV-2 (COVID-19) ORF3b Protein Antibodies: SARS-CoV-2 (COVID-19) ORF3b Antibody, 9277. A direct ELISA was performed using SARS-CoV-2 ORF3b recombinant protein (10-005) as coating antigen and the anti-SARS-CoV-2 (COVID-19) ORF3b antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/mL

Figure 2 Western Blot Validation with SARS-CoV-2 (COVID-19) ORF3b ProteinLoading: 30 ng per lane of SARS-CoV-2 (COVID-19) ORF3b recombinant protein (10-005).Antibodies: SARS-CoV-2 (COVID-19) ORF3b, 9277, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: 0.1 &956,g/mL and Lane 2: 0.2 &956,g/mL