SARS-CoV-2 (COVID-19) ORF8 Antibody, Unconjugated, Rabbit, Polyclonal
Catalog Number:
PRS-9289
- Images (2)
| Article Name: | SARS-CoV-2 (COVID-19) ORF8 Antibody, Unconjugated, Rabbit, Polyclonal |
| Biozol Catalog Number: | PRS-9289 |
| Supplier Catalog Number: | 9289 |
| Alternative Catalog Number: | PRS-9289-0.02,PRS-9289-0.1 |
| Manufacturer: | ProSci |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, WB |
| Species Reactivity: | Virus |
| Immunogen: | Anti-SARS-CoV-2 (COVID-19) ORF8 antibody (9289) was raised against a peptide corresponding to 13 amino acids near the central domain of SARS-CoV-2 (COVID-19) ORF8 protein. The immunogen is located within 30-80 amino acids of the SARS-CoV-2 (COVID-19) ORF8 protein. |
| Conjugation: | Unconjugated |
| Alternative Names: | Non-structural protein 8, ORF8, ORF8 protein, covid-19, ns8, sars-cov-2 |
| Application Dilute: | Optimal dilutions for each application to be determined by the researcher. |
| Application Notes: | WB: 1 µg/mLAntibody validated: SARS-CoV-2 (COVID-19) ORF8 antibody can detect 2 ng of free peptide at 1 µg/mL in ELISA. It can detect SARS-CoV-2 ORF8 recombinant protein by WB. All other applications and species not yet tested. |
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Figure 2 ELISA Validation Antibodies: SARS-CoV-2 (COVID-19) ORF8 Antibody, 9289. A direct ELISA was performed using SARS-CoV-2 ORF8 immunogen peptide (9289P)) as coating antigen and the anti-SARS-CoV-2 (COVID-19) ORF8 antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL |
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Figure 1 Western Blot Validation with SARS-CoV-2 (COVID-19) ORF8 ProteinLoading: 30 ng per lane of SARS-CoV-2 (COVID-19) ORF8 recombinant protein (10-436).Antibodies: SARS-CoV-2 (COVID-19) ORF8, 9289, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: 1 &956,g/mL and Lane 2: 2 &956,g/mL |
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