SARS-CoV-2 Spike P681H Antibody (Alpha, Mu Variant), Unconjugated, Rabbit, Polyclonal

Article Name:	SARS-CoV-2 Spike P681H Antibody (Alpha, Mu Variant), Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number:	PRS-9359
Supplier Catalog Number:	9359
Alternative Catalog Number:	PRS-9359-0.02,PRS-9359-0.1
Manufacturer:	ProSci
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, WB
Species Reactivity:	Virus
Immunogen:	Anti-SARS-CoV-2 (COVID-19) P681H Mutant Specific Spike P681H Antibody (9359) was raised against a peptide corresponding to 12 amino acids near the center of SARS-CoV-2 UK variant (B.1.1.7) Spike protein. The immunogen is located within 650-700 amino acids of SARS-CoV-2 UK variant (B.1.1.7) Spike protein.
Conjugation:	Unconjugated
Alternative Names:	SARS-CoV-2 (COVID-19) Spike Antibody: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Spike protein, Surface Glycoprotein, sars-cov-2

Clonality:	Polyclonal
Concentration:	1 mg/mL
NCBI:	43740568
UniProt:	P0DTC2
Buffer:	SARS-CoV-2 (COVID-19) P681H Mutant Specific Spike antibody is supplied in PBS containing 0.02% sodium azide.
Form:	Liquid

Application Dilute:	Optimal dilutions for each application to be determined by the researcher.
Application Notes:	WB: 1 µg/mL.Antibody validated: Western Blot in human samples. Anti-SARS-CoV-2 (COVID-19) P681H Mutant Specific Spike antibody can specifically detect SARS-CoV-2 UK Variant (B.1.1.7) Spike S1 protein, but not SARS-CoV-2 WT Spike S1 protein by ELISA. It can also detect mutant peptide (681H), but not WT peptide (681P). All other applications and species not yet tested.

	Figure 2 ELISA Validation of P681H Mutant Specific Spike Antibodies with SARS-CoV-2 UK Variant Spike S1 Protein Coating Antigen: SARS-CoV-2 spike S1 proteins, including WT and UK variant (B.1.1.7), 1 &956,g/mL, incubate at 4 &730,C overnight.Detection Antibodies: SARS-CoV-2 UK Variant Spike antibody, 9359, dilution: 0.1-1000 ng/mL, incubate at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr.SARS-CoV-2 P681H Mutant Specific Spike antibody (9359) can specifically detect UK variant spike S1 protein, but not WT spike S1 protein (10-300).
	Figure 4 ELISA Validation of P681H Mutant Specific Spike Antibodies with Mutant and WT Peptide Coating Antigen: SARS-CoV-2 spike S1 peptides, including WT and UK variant (B.1.1.7), 1 &956,g/mL, incubate at 4 &730,C overnight.Detection Antibodies: SARS-CoV-2 P681H Mutant Specific Spike antibody, 9091, dilution: 0.1-1000 ng/mL, incubate at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr.Immunogen region of antibody 9091 includes site 681P that was mutated in B.1.1.7. SARS-CoV-2 P681H Mutant Specific Spike antibody (9359) can specifically detect UK variant spike S1 peptide (681H), but not WT peptide (681P).
	Figure 1 ELISA Validation of Alpha Variant Spike Antibodies with Spike S1 Protein of SARS-CoV-2 Variants Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), and mu variant (B.1.621), 1 &956,g/mL, incubate at 4 &730,C overnight.Detection Antibodies: SARS-CoV-2 Alpha Variant Spike antibody, 9359, dilution: 200-1000 ng/mL, incubate at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr.SARS-CoV-2 alpha variant spike antibody (9359) can specifically detect alpha variant spike S1 protein, but not spike S1 protein of WT and other tested variants by ELISA.
	Figure 3 WB Validation of P681H Mutant Specific Spike Antibodies with SARS-CoV-2 UK Variant Spike S1 Protein Loading: 50 ng of SARS-CoV-2 spike S1 proteins, including WT and UK variant (B.1.1.7).Detection Antibodies: SARS-CoV-2 P681H Mutant Specific Spike antibody, 9359, 1 &956,g/mL, incubate at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr.SARS-CoV-2 P681H Mutant Specific Spike antibody (9359) can specifically detect UK variant spike S1 protein, but not WT spike S1 protein (10-300).