SARS-CoV-2 (COVID-19) Delta Variant Spike S1 (His-Avi Tag) Recombinant Protein

Product Overview

• Article Name: SARS-CoV-2 (COVID-19) Delta Variant Spike S1 (His-Avi Tag) Recombinant Protein
• Biozol Catalog Number: PRS-95-127
• Supplier Catalog Number: 95-127
• Alternative Catalog Number: PRS-95-127-0.05
• Manufacturer: ProSci
• Category: Proteine/Peptide
• Application: ELISA, SDS-PAGE, WB
• Alternative Names: Delta variant spike RBD, S glycoprotein RBD, S protein RBD, Spike glycoprotein Receptor-binding domain, Spike protein RBD, covid-19, sars-cov-2

Product Properties

• Molecular Weight: 120 kD
• NCBI: 43740578
• UniProt: P0DTC1
• Buffer: PBS (pH 7.4).
• Source: HEK293 cells
• Purity: > 90% as determined by Bis-Tris PAGE
• Form: Liquid

Application Notes

• Application Notes: This recombinant protein can be used for WB and ELISA. For research use only.

Images

Figure 1 ELISA Binding Assay of ACE2 and SARS-CoV-2 (COVID-19) Delta Variant Spike S1 Recombinant Protein Coating Antigen: SARS-CoV-2 Delta variant (B.1.671.2) spike S1 protein, 1 &956,g/mL, incubated at 4 &730,C overnight.Detection Antibodies: ACE2 recombinant protein, 10-120, dilution: 0.3- 1000 ng/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-human HRP at 1:10,000, incubate at RT for 1 hr.

Figure 3 ELISA Validation of Delta Variant Spike S1 Protein with SARS-CoV-2 Delta Variant Spike Antibodies Coating Antigen: SARS-CoV-2 spike S1 proteins, including WT and Delta variant (B.1.671.2), 5 &956,g/mL, incubated at 4 &730,C overnight.Detection Antibodies: SARS-CoV-2 Delta variant spike 156-157EFdel antibody, 9689, and spike 156-157EF antibody, 9685, dilution: 200 and 1000 ng/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr.SARS-CoV-2 Delta Variant Spike S1 protein (95-127), but not WT spike S1 protein (10-300), was specifically detected by Delta Variant spike antibody (9689), while the corresponding WT antibody (9685) detected spike S1 protein of WT, but not delta variant.

Figure 4 ELISA Validation of Delta Variant Spike S1 Protein with SARS-CoV-2 Delta Variant Spike Antibodies Coating Antigen: SARS-CoV-2 spike S1 proteins, including WT and Delta variant (B.1.671.2), 5 &956,g/mL, incubated at 4 &730,C overnight.Detection Antibodies: SARS-CoV-2 Delta variant spike P681R antibodies, PM-9677 and PM9680, dilution: 200 and 1000 ng/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr.SARS-CoV-2 Delta Variant Spike S1 protein (95-127), but not WT spike S1 protein (10-300), was specifically detected by Delta Variant spike antibodies.

Figure 6 ELISA Validation of Delta Variant Spike S1 Protein with SARS-CoV-2 Spike S1 Antibodies Coating Antigen: SARS-CoV-2 spike S1 proteins, including WT and Delta variant (B.1.671.2), 5 &956,g/mL, incubated at 4 &730,C overnight.Detection Antibodies: SARS-CoV-2 Spike S1 antibody, 9083, dilution: 0.3-1000 ng/mL, incubate at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr.Antibody 9083 does not distinguish between WT and Delta.

Figure 2 SDS-PAGE Validation of SARS-CoV-2 (COVID-19) Delta Variant Spike S1 Recombinant Protein Loading: 1 &956,g SARS-CoV-2 (COVID-19) Delta variant Spike S1 recombinant protein, 95-127.Observed: 120kD

Figure 5 WB Validation of Delta Variant Spike S1 Protein with SARS-CoV-2 Delta Variant Spike Antibodies Loading: 20 ng of SARS-CoV-2 spike S1 proteins, including WT and Delta variant (B.1.671.2).Detection Antibodies: SARS-CoV-2 Delta variant spike antibody, 9689, 1 &956,g/mL, incubate at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr.SARS-CoV-2 Delta Variant S1 protein (95-127), but not WT spike S1 protein (10-300), was specifically detected by Delta Variant spike antibody (9689).