PGAM5 (CT) Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: PGAM5 (CT) Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-9517
• Supplier Catalog Number: 9517
• Alternative Catalog Number: PRS-9517-0.02,PRS-9517-0.1
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Anti-PGAM5 antibody (9517) was raised against a peptide corresponding to 14 amino acids near the carboxyl terminus of human PGAM5. The immunogen is located within the last 50 amino acids of PGAM5.
• Conjugation: Unconjugated
• Alternative Names: Serine/threonine-protein phosphatase PGAM5 mitochondrial, Bcl-XL-binding protein v68, Phosphoglycerate mutase family member 5

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/mL
• Molecular Weight: Predicted: 32kD Observed: 32 kD
• NCBI: 192111
• UniProt: Q96HS1
• Buffer: PGAM5 Antibody is supplied in PBS containing 0.02% sodium azide
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 0.5 µg/mL, IF: 10-20 µg/mL. Antibody validated: Western Blot in human, mouse, and rat samples, Immunofluorescence in human, mouse, and rat samples. All other applications and species not yet tested.

Images

Figure 5 Immunofluorescence Validation of PGAM5 in Human LiverImmunofluorescent analysis of 4% paraformaldehyde-fixed human liver labeling PGAM5 with 9517 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Figure 6 Immunofluorescence Validation of PGAM5 in Mouse TestisImmunofluorescent analysis of 4% paraformaldehyde-fixed mouse testis labeling PGAM5 with 9517 at 10 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Figure 7 Immunofluorescence Validation of PGAM5 in Rat TestisImmunofluorescent analysis of 4% paraformaldehyde-fixed rat testis labeling PGAM5 with 9517 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Figure 1 Western Blot Validation in Mouse and Rat Cell Lines Loading: 15 &956,g of lysates per lane. Antibodies: PGAM5, 9517 0.5 &956,g/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.

Figure 2 Western Blot Validation in Human Cell Lines Loading: 15 &956,g of lysates per lane. Antibodies: PGAM5, 9517 0.5 &956,g/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.

Figure 3 Western Blot Validation in Mouse Tissues Loading: 15 &956,g of lysates per lane. Antibodies: PGAM5, 9517 0.5 &956,g/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.

Figure 4 Western Blot Validation in Rat Tissues Loading: 15 &956,g of lysates per lane. Antibodies: PGAM5, 9517 0.5 &956,g/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.