IFI16 (CT) Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: IFI16 (CT) Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-9641
• Supplier Catalog Number: 9641
• Alternative Catalog Number: PRS-9641-0.02,PRS-9641-0.1
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Anti-IFI16 antibody (9641) was raised against a peptide corresponding to 16 amino acids near the carboxyl terminus of human IFI16. The immunogen is located within the last 50 amino acids of IFI16.
• Conjugation: Unconjugated
• Alternative Names: IFI16 Antibody: Gamma-interferon-inducible protein 16, Ifi-16, Interferon-inducible myeloid differentiation transcriptional activator, IFNGIP1

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/mL
• Molecular Weight: Predicted: 88kD Observed: 88 kD
• NCBI: 3428
• UniProt: Q16666
• Buffer: IFI16 Antibody is supplied in PBS containing 0.02% sodium azide.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 2 µg/mL, IF: 20 µg/mL, IHC: 1 µg/mL. Antibody validated: Western Blot in human, mouse, and rat samples, Immunofluorescence in human samples, Immunohistochemistry in human, mouse and rat samples. All other applications and species not yet tested.

Images

Figure 4 Immunofluorescence Validation of IFI16 in Human Molt4 CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed Molt4 cells labeling IFI16 with 9641 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Figure 5 Immunohistochemistry Validation of IFI16 in Human TestisImmunohistochemical analysis of paraffin-embedded human testis tissue using anti-IFI16 antibody (9641) at 1 &956,g/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Figure 6 Immunohistochemistry Validation of IFI16 in Mouse LiverImmunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-IFI16 antibody (9641) at 1 &956,g/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Figure 7 Immunohistochemistry Validation of IFI16 in Rat TestisImmunohistochemical analysis of paraffin-embedded rat testis tissue using anti-IFI16 antibody (9641) at 1 &956,g/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Figure 1 WB Validation in Human and Rat Cell Lines Loading: 15 &956,g of lysate per lane. Antibodies: IFI16 9641, 2 &956,g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.

Figure 2 Western Blot Validation in Mouse Tissues Loading: 15 &956,g of lysates per lane. Antibodies: IFI16 9641, 2 &956,g/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.

Figure 3 Western Blot Validation in Rat TestisLoading: 15 &956,g of lysate per lane. Antibodies: IFI16 9641, 2 &956,g/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.