SARS-CoV-2 (COVID-19) Spike G142D delta143-145VYY Antibody (Omicron), Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: SARS-CoV-2 (COVID-19) Spike G142D delta143-145VYY Antibody (Omicron), Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-9793
• Supplier Catalog Number: 9793
• Alternative Catalog Number: PRS-9793-0.02,PRS-9793-0.1
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, ICC, IF, WB
• Species Reactivity: Virus
• Immunogen: Anti-SARS-CoV-2 Omicron variant specific Spike (G142D delta143-145VYY) antibody(9793) was raised against a peptide corresponding to 12 amino acids near the amino terminal domain of SARS-CoV-2 Omicron variant (B.1.1.529) Spike protein. The immunogen is located within 130-180 amino acids of SARS-CoV-2 Omicron variant (B.1.1.529) Spike protein.
• Conjugation: Unconjugated
• Alternative Names: Omicron Variant, SARS-CoV-2 Omicron Variant Spike antibody: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Spike protein, Surface Glycoprotein, covid-19, sars-cov-2

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/mL
• NCBI: 43740568
• UniProt: P0DTC2
• Buffer: Anti-SARS-CoV-2 Omicron Variant Spike antibody is supplied in PBS containing 0.02% sodium azide.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 2 µg/mL, ICC: 1 µg/mL, IF: 10 µg/mL. Antibody validated: Western Blot, Immunocytochemistry and Immunofluorescence staining in human samples. Anti-SARS-CoV-2 Omicron variant Spike antibody specifically detects Spike S1 protein of Omicron Variant (B.1.1.529), but not WT and other variant by ELISA and WB. Other applications and species not yet tested.

Images

Figure 3 SARS-CoV-2 Omicron Variant Spike Antibodies Specifically Detect Omicron Variant Spike S1 Protein in an ELISA Coating Antigen: SARS-CoV-2 spike S1 proteins of WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), and omicron variant (B.1.1.529), 1 &956,g/mL, incubated at 4 &730,C overnight.Detection Antibodies: SARS-CoV-2 Omicron Variant Spike antibody, 9793, dilution: 200 and 1000 ng/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubated at RT for 1 hr.

Figure 5 ELISA Validation of Omicron Variant Spike S1 Antibodies with SARS-CoV-2 Omicron Variant Spike S1 Protein Coating Antigen: SARS-CoV-2 spike S1 proteins, WT and Omicron variant, 1 &956,g/mL, incubated at 4 &730,C overnight.Detection Antibodies: SARS-CoV-2 Omicron spike antibody, 9793, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubated at RT for 1 hr.SARS-CoV-2 Omicron spike antibody specifically detects spike S1 protein of Omicron variant (95-129), but not WT (10-300).

Figure 2 Immunofluorescence Validation of Omicron Variant Spike Specific Antibody on a Nasal Swab Sample from a Patient Infected with the Omicron Variant. Immunofluorescent analysis of 4% paraformaldehyde-fixed patient nasal swab sample labeling spike with 9793 and nucleocapsid with 35-722 at 10 &956,g/mL for each, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution for spike (9793,green) and goat anti-mouse IgG secondary antibody at 1/500 dilution for nucleocapsid (35-722, red) with DAPI counter staining (blue).

Figure 1 Immunohistochemistry Validation of Omicron Variant Spike Specific Antibody on a Nasal Swab Sample from a Omicron COVID-19 Patient. Immunohistochemical analysis of 4% paraformaldehyde-fixed cells from a nasal swab from a patient infected with the Omicron variant stained with Omicron specific antibody (9793) at 1 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Figure 4 WB Validation of of Omicron Variant Spike Antibodies with SARS-CoV-2 Omicron Variant Spike S1 Protein Loading: 30 ng of SARS-CoV-2 spike S1 proteins, including WT, Delta variant (B.1.617.2) and Omicron variant (B.1.1.529).Detection Antibodies: SARS-CoV-2 Omicron Variant Spike antibody, 9793, 2 &956,g/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubated at RT for 1 hr.SARS-CoV-2 Omicron Variant Spike antibody (9793) specifically detects Omicron variant spike S1 protein (95-129), but not spike S1 protein of WT (10-300) and Delta (95-127).

Figure 6 WB Validation of of Omicron Variant Spike Antibodies with SARS-CoV-2 Variant Spike S1 Proteins Loading: 30 ng of SARS-CoV-2 spike S1 proteins, including WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), and omicron variant (B.1.1.529),Detection Antibodies: SARS-CoV-2 Omicron Variant Spike antibody, 9793, 2 &956,g/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubated at RT for 1 hr.SARS-CoV-2 Omicron Variant Spike antibody (9793) specifically detects Omicron variant spike S1 protein (95-129), but not spike S1 protein of WT (10-300) and all other variants.