SARS-CoV-2 (COVID-19) Spike Q493R G496S Q498R N501Y Y505H Antibody (Omicron), Unconjugated, Rabbit, Polyclonal

Article Name:	SARS-CoV-2 (COVID-19) Spike Q493R G496S Q498R N501Y Y505H Antibody (Omicron), Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number:	PRS-9805
Supplier Catalog Number:	9805
Alternative Catalog Number:	PRS-9805-0.02,PRS-9805-0.1
Manufacturer:	ProSci
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, IF, NeA, WB
Species Reactivity:	Virus
Immunogen:	Anti-SARS-CoV-2 Spike Q493R G496S Q498R N501Y Y505H (Omicron) antibody(9805) was raised against a peptide corresponding to 20 amino acids in the receptor binding domain of SARS-CoV-2 Omicron variant (B.1.1.529) Spike protein. The immunogen is located within 480-530 amino acids of SARS-CoV-2 Omicron variant (B.1.1.529) Spike protein.
Conjugation:	Unconjugated
Alternative Names:	Omicron Variant, SARS-CoV-2 Omicron Variant Spike antibody: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Spike protein, Surface Glycoprotein, covid-19, sars-cov-2

Clonality:	Polyclonal
Concentration:	1 mg/mL
NCBI:	43740568
UniProt:	P0DTC2
Buffer:	Anti-SARS-CoV-2 Omicron Variant Spike antibody is supplied in PBS containing 0.02% sodium azide.
Form:	Liquid

Application Dilute:	Optimal dilutions for each application to be determined by the researcher.
Application Notes:	WB: 2 µg/mL, IF: 20 µg/mL, Neut: 0.1-10 µg/mL Antibody validated: Western Blot and Immunofluorescence staining in human samples, Neutralization in Omicron pseudovirus samples. Anti-SARS-CoV-2 Omicron variant Spike antibody specifically detects Spike S1 protein of Omicron Variant (B.1.1.529), but not WT and other variant by ELISA and WB. All other applications and species not yet tested.

	Figure 3 SARS-Cov-2 Omicron Variant Spike Antibodies Specifically Detect Omicron Variant Spike S1 Protein in an ELISA Coating Antigen: SARS-CoV-2 spike S1 proteins of WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), and omicron variant (B.1.1.529), 1 &956,g/mL, incubated at 4 &730,C overnight.Detection Antibodies: SARS-CoV-2 Omicron Variant Spike antibody, 9805, 1 &956,g/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubated at RT for 1 hr.
	Figure 4 SARS-Cov-2 Omicron Variant Spike Antibodies Specifically Detect Omicron Variant Spike RBD Protein in an ELISA Coating Antigen: SARS-CoV-2 spike RBD proteins of WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), and omicron variant (B.1.1.529), 1 &956,g/mL, incubated at 4 &730,C overnight.Detection Antibodies: SARS-CoV-2 Omicron Variant Spike antibody, 9805, 1 &956,g/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubated at RT for 1 hr.
	Figure 1 Immunofluorescence Validation of Spike RBD in the Nasal Swab Sample of Omicron Variant COVID-19 Patient. Immunofluorescent analysis of 4% paraformaldehyde-fixed patient nasal swab sample labeling spike RBD with 9805 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
	Figure 2 Neutralization Assay of Omicron Variant Pseudovirus by SARS-CoV-2 Omicron Variant Spike RBD Antibody Neutralization antibodies: Anti-SARS-CoV-2 Spike RBD antibodies, 9805, dilution: 0.3 - 30,000 ng/mL.Pseudovirus: SARS-CoV-2 Omicron Spike Pseudovirus, 95-201.Infected Cells: ACE2-overexpressing 293T cellsDiluted antibody was incubated with Omicron pseudovirus at 37 &730,C for 1 hr. ACE2-overexpressing 293T cells were added in each well and incubated at 37 &730,C for 48 hrs. Luminescence reporter reagent was added in each well and the final results were read with the luminescence plate reader.Percent inhibition is calculated based on the RLU value.
	Figure 5 WB Validation of of Omicron Variant Spike Antibodies with SARS-CoV-2 Variant Spike S1 Proteins Loading: 30 ng of SARS-CoV-2 spike S1 proteins, including WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), and omicron variant (B.1.1.529),Detection Antibodies: SARS-CoV-2 Omicron Variant Spike antibody, 9805, 2 &956,g/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubated at RT for 1 hr.SARS-CoV-2 Omicron Variant Spike antibody (9805) specifically detects Omicron variant spike S1 protein (95-129), but not spike S1 protein of WT (10-300) and all other variants.