CD4 Antibody [9H5A8], IgG1, Unconjugated, Mouse, Monoclonal

Product Overview

• Article Name: CD4 Antibody [9H5A8], IgG1, Unconjugated, Mouse, Monoclonal
• Biozol Catalog Number: PRS-PM-5201
• Supplier Catalog Number: PM-5201
• Alternative Catalog Number: PRS-PM-5201-0.02,PRS-PM-5201-0.1
• Manufacturer: ProSci
• Host: Mouse
• Category: Antikörper
• Application: ELISA, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Mouse monoclonal CD4 antibody was raised against a 193 amino acid recombinant protein from near the amino terminus of human CD4.
• Conjugation: Unconjugated
• Alternative Names: CD4 Antibody [9H5A8] : CD4mut

Product Properties

• Clonality: Monoclonal
• Concentration: 1 mg/mL
• Clone Designation: [9H5A8]
• Molecular Weight: Predicted: 51kDObserved: 51-57 kD (Post-modyfication: 2-4 N-linked glycosylations)
• Isotype: IgG1
• NCBI: 920
• UniProt: P01730
• Buffer: CD4 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 0.5-4 µg/mL, IHC: 5 µg/mL.Antibody validated: Western Blot in human, mouse and rat samples, Immunohistochemistry in human samples. All other applications and species not yet tested.

Images

Figure 8 Immunohistochemistry Validation of CD4 in Human Thymus Tissue Immunohistochemical analysis of paraffin-embedded human thymus tissue using anti-CD4 antibody (PM-5201) at 5 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-mouse IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Figure 1 Western Blot Validation with Human Recombinant ProteinLoading: 5ng (Lane 1), 25ng (Lane 2) and 100ng (Lane 3) of human CD4 recombinant protein.Antibodies: CD4 PM-5201 (1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.Observed at around 27kD.

Figure 2 Western Blot Validation in Human Thymus Tissue LysateLoading: 15 &956,g of lysates per lane.Antibodies: CD4 PM-5201 (Lane 1: 0.5 &956,g/mL, Lane 2: 1 &956,g/mL and Lane 3: 2 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.

Figure 3 Western Blot Validation in Human Jurkat LysateLoading: 15 &956,g of lysates per lane.Antibodies: CD4 PM-5201 (Lane 1: 1 &956,g/mL, Lane 2: 2 &956,g/mL and Lane 3: 4 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.

Figure 4 Western Blot Validation in Human, Mouse and Rat Tissue LysateLoading: 15 &956,g of lysates per lane.Antibodies: CD4 PM-5201 (1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.

Figure 5 Western Blot Validation in Human Cell Lines Loading: 15 &956,g of lysates per lane.Antibodies: CD4 PM-5201 (4 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.

Figure 6 Western Blot Validation in Mouse Spleen Tissue LysateLoading: 15 &956,g of lysates per lane.Antibodies: CD4 PM-5201 (Lane 1: 1 &956,g/mL, Lane 2: 2 &956,g/mL and Lane 3: 4 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.

Figure 7 Western Blot Validation in Rat Lung Tissue LysateLoading: 15 &956,g of lysates per lane.Antibodies: CD4 PM-5201 (Lane 1: 0.5 &956,g/mL, Lane 2: 1 &956,g/mL and Lane 3: 2 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.