SARS-CoV-2 (COVID-19) Spike 681P Antibody [8G10B1], IgG3, Unconjugated, Mouse, Monoclonal

Product Overview

• Article Name: SARS-CoV-2 (COVID-19) Spike 681P Antibody [8G10B1], IgG3, Unconjugated, Mouse, Monoclonal
• Biozol Catalog Number: PRS-PM-9366
• Supplier Catalog Number: PM-9366
• Alternative Catalog Number: PRS-PM-9366-0.02,PRS-PM-9366-0.1
• Manufacturer: ProSci
• Host: Mouse
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Virus
• Immunogen: Anti-SARS-CoV-2 (COVID-19) Spike 681P antibody (PM-9366) was raised against a peptide corresponding to 12 amino acids near the center of SARS-CoV-2 Spike protein. The immunogen is located within 650-700 amino acids of the SARS-CoV-2 Spike protein.
• Conjugation: Unconjugated
• Alternative Names: SARS-CoV-2 Spike 681P antibody: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Spike protein, Surface Glycoprotein, covid-19, sars-cov-2

Product Properties

• Clonality: Monoclonal
• Concentration: 1 mg/mL
• Clone Designation: [8G10B1]
• Isotype: IgG3
• NCBI: 43740568
• UniProt: P0DTC2
• Buffer: Anti-SARS-CoV-2 Spike 681P antibody is supplied in PBS containing 0.02% sodium azide.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1 µg/mL.Antibody validated: Western Blot in human samples. Anti-SARS-CoV-2 Spike 681P antibody can detect SARS-CoV-2 WT Spike S1 protein, but not SARS-CoV-2 Alpha Variant (B.1.1.7, UK) S1 protein by ELISA and WB. It can also detect WT peptide (681P), but not mutant peptide (681H). All other applications and species not yet tested.

Images

Figure 1 ELISA Validation of Spike 681P Antibodies with SARS-CoV-2 Spike S1 Protein Coating Antigens: SARS-CoV-2 spike S1 proteins: WT and alpha variant (B.1.1.7, UK), 2 &956,g/mL, incubated at 4 &730,C overnight.Detection Antibodies: SARS-CoV-2 Spike 681P antibody, PM-9366, dilution: 1-3000 ng/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.SARS-CoV-2 spike 681P antibody (PM-9366) detected WT spike S1 protein (10-300), but not alpha variant spike S1 protein by ELISA.

Figure 3 ELISA Validation of Spike 681P Antibodies with Mutant and WT Peptide Coating Antigen: SARS-CoV-2 spike S1 peptides: WT (9091P) and alpha variant (B.1.1.7, UK) (9359P), 1 &956,g/mL, incubate at 4 &730,C overnight.Detection Antibodies: SARS-CoV-2 681P antibody, PM-9366, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.SARS-CoV-2 spike 681P antibody (PM-9366) detected WT peptide (681P, 9091P), but not alpha variant spike S1 peptide (681H, 9359P).

Figure 2 WB Validation of Spike 681P Antibodies with SARS-CoV-2 Spike S1 Protein Loading: 30 ng of SARS-CoV-2 spike S1 proteins: WT and alpha variant (B.1.1.7, UK).Detection Antibodies: SARS-CoV-2 Spike 681P antibody, PM-9366, 1 &956,g/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.SARS-CoV-2 spike 681P antibody (PM-9366) detected WT spike S1 protein (10-300), but not alpha variant spike S1 protein by WB.