SARS-CoV-2 Spike P681H Antibody [7A4D12](Alpha, Mu Variant), Unconjugated, Mouse, Monoclonal

Product Overview

• Article Name: SARS-CoV-2 Spike P681H Antibody [7A4D12](Alpha, Mu Variant), Unconjugated, Mouse, Monoclonal
• Biozol Catalog Number: PRS-PM-9374
• Supplier Catalog Number: PM-9374
• Alternative Catalog Number: PRS-PM-9374-0.02,PRS-PM-9374-0.1
• Manufacturer: ProSci
• Host: Mouse
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Virus
• Immunogen: Anti-SARS-CoV-2 (COVID-19) P681H Mutant Specific Spike P681H antibody (PM-9374) was raised against a peptide corresponding to 12 amino acids near the center of SARS-CoV-2 Alpha variant (B.1.1.7) Spike protein. The immunogen is located within 650-700 amino acids of SARS-CoV-2 Alpha Variant (B.1.1.7, UK) Spike protein.
• Conjugation: Unconjugated
• Alternative Names: SARS-CoV-2 (COVID-19) P681H Mutant Specific Spike antibody: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Spike protein, Surface Glycoprotein, sars-cov-2

Product Properties

• Clonality: Monoclonal
• Concentration: 1 mg/mL
• Clone Designation: [7A4D12]
• NCBI: 43740568
• UniProt: P0DTC2
• Buffer: SARS-CoV-2 (COVID-19) P681H Mutant Specific Spike antibody is supplied in PBS containing 0.02% sodium azide.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1 µg/mL.Antibody validated: Western Blot in human samples. Anti-SARS-CoV-2 (COVID-19) P681H Mutant Specific Spike antibody can specifically detect SARS-CoV-2 Alpha Variant (B.1.1.7, UK) S1 protein, but not SARS-CoV-2 WT Spike S1 protein by ELISA and WB. It can also detect mutant peptide (681H), but not WT peptide (681P). All other applications and species not yet tested.

Images

Figure 2 ELISA Validation of P681H Mutant Specific Spike Antibodies with SARS-CoV-2 Alpha Variant Spike S1 Protein Coating Antigen: SARS-CoV-2 spike S1 proteins, including WT and alpha variant (B.1.1.7, UK), 1 &956,g/mL, incubate at 4 &730,C overnight.Detection Antibodies: SARS-CoV-2 Alpha Variant Spike antibody, PM-9374, dilution: 0.3-1000 ng/mL, incubate at RT for 1 hr.Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr.SARS-CoV-2 P681H Mutant Specific Spike antibody (PM-9374) can specifically detect alpha variant spike S1 protein, but not WT spike S1 protein (10-300) by ELISA.

Figure 4 ELISA Validation of P681H Mutant Specific Spike Antibodies with Mutant and WT Peptide Coating Antigen: SARS-CoV-2 spike S1 peptides, including WT (9091P) and alpha variant (B.1.1.7, UK) (9359P), 1 &956,g/mL, incubate at 4 &730,C overnight.Detection Antibodies: SARS-CoV-2 P681H Mutant Specific Spike antibody, PM-9374, dilution: 0.1-1000 ng/mL, incubate at RT for 1 hr.Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr.SARS-CoV-2 P681H Mutant Specific Spike antibody (PM-9374) can specifically detect alpha variant spike S1 peptide (681H, 9359P), but not WT peptide (681P, 9091P).

Figure 1 ELISA Validation of Alpha Variant Spike Antibodies with Spike S1 Protein of SARS-CoV-2 Variants Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), and omicron variant (B.1.1.529), 1 &956,g/mL, incubate at 4 &730,C overnight.Detection Antibodies: SARS-CoV-2 Alpha Variant Spike antibody, PM-9374, dilution: 200-1000 ng/mL, incubate at RT for 1 hr.Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr.SARS-CoV-2 alpha variant spike antibody (PM-9374) can specifically detect alpha variant spike S1 protein, but not spike S1 protein of WT and other tested variants by ELISA.

Figure 3 WB Validation of P681H Mutant Specific Spike Antibodies with SARS-CoV-2 Alpha Variant Spike S1 Protein Loading: 50 ng of SARS-CoV-2 spike S1 proteins, including WT and alpha variant (B.1.1.7, UK).Detection Antibodies: SARS-CoV-2 P681H Mutant Specific Spike antibody, PM-9374, 1 &956,g/mL, incubate at RT for 1 hr.Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr.SARS-CoV-2 P681H Mutant Specific Spike antibody (PM-9374) can specifically detect alpha variant spike S1 protein, but not WT spike S1 protein (10-300) by WB.