Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody (PM-9429) was raised against a peptide corresponding to 20 amino acids near the carboxy terminus of SARS-CoV-2 (COVID-19) Spike glycoprotein. The immunogen is located within the last 50 amino acids of SARS-CoV-2 (COVID-19) Spike protein.
SARS-CoV-2 (COVID-19) Spike Antibody is supplied in PBS containing 0.02% sodium azide.
Form:
Liquid
Application Dilute:
Optimal dilutions for each application to be determined by the researcher.
Application Notes:
WB: 0.2 µg/mL, IF: 20 µg/mL. IHC: 0.5 µg/mLAntibody validated: Immunofluorescence and Western blot in human samples. Immunohistochemistry in COVID-19 patient samples. SARS-CoV-2 (COVID-19) Spike S2 antibody can be used for the detection of SARS-CoV-2 (COVID-19) Spike full length protein in ELISA. It will detect 4 ng of free peptide at 1 µg/mL. PM-9429 cannot be used for detection of SARS-CoV-2/SARS-CoV Spike S1, Spike ECD, Spike S2 ECD proteins. All other applications and species not yet tested.
Figure 4 ELISA TestAntibodies: SARS-CoV-2 (COVID-19) Spike S2 antibody, PM-9429 (1 &956,g/mL). A direct ELISA was performed using immunogen or control peptide as coating antigen and the anti-SARS-CoV-2 (COVID-19) Spike antibody as the capture antibody. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5.000 dilution. Detection range is from 0.3 ng/mL to 1,000 ng/mL.
Figure 3 Immunofluorescence Validation of SARS-CoV-2 (COVID-19) Spike in 293T CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed 293T cells labeling SARS-CoV-2 (COVID-19) Spike S2 with PM-9429 at 20 &956,g/mL, followed by goat anti-mouse IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
Figure 1 Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) Spike in COVID-19 Patient Lung Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti-SARS-CoV-2 (COVID-19) Spike S2 antibody (PM-9429, 0.5 &956,g/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,. A goat anti-mouse IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong spike protein signal was observed in macrophages of COVID-19 patient lung, but not in non-COVID-19 patient lung.
Figure 2 Overexpression Validation in Spike Transfected 293 Cells Loading: 5 µg per lane of 293 cell lysate.Antibodies: SARS-CoV-2 (COVID-19) Spike S2, PM-9429, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.Lane 1: 0.1 &956,g/mL andLane 2: 0.2 &956,g/mL
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