SARS-CoV-2 (COVID-19) Spike P26S Antibody [5G12G11] (Gamma Variant), IgG1, Unconjugated, Mouse, Monoclonal

Product Overview

• Article Name: SARS-CoV-2 (COVID-19) Spike P26S Antibody [5G12G11] (Gamma Variant), IgG1, Unconjugated, Mouse, Monoclonal
• Biozol Catalog Number: PRS-PM-9590
• Supplier Catalog Number: PM-9590
• Alternative Catalog Number: PRS-PM-9590-0.02,PRS-PM-9590-0.1
• Manufacturer: ProSci
• Host: Mouse
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Virus
• Immunogen: Anti-SARS-CoV-2 Spike P26S (Gamma Variant) antibody (PM-9590) was raised against a peptide corresponding to 11 amino acids near the amino terminus of SARS-CoV-2 Gamma variant (P.1) Spike protein. The immunogen is located within the first 50 amino acids of SARS-CoV-2 Gamma variant (P.1) Spike protein.
• Conjugation: Unconjugated
• Alternative Names: SARS-CoV-2 Spike 156-157EF antibody: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Spike protein, Surface Glycoprotein, covid-19, sars-cov-2

Product Properties

• Clonality: Monoclonal
• Concentration: 1 mg/mL
• Clone Designation: [5G12G11]
• Isotype: IgG1
• NCBI: 43740568
• UniProt: P0DTC2
• Buffer: SARS-CoV-2 Spike P26S (Gamma Variant) antibody is supplied in PBS containing 0.02% sodium azide.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1 µg/mL.Antibody validated: Western Blot in human samples. Anti-SARS-CoV-2 Spike P26S (Gamma Variant) antibody specifically detects SARS-CoV-2 Gamma Variant (P.1) Spike S1 protein, but not SARS-CoV-2 WT and other variant Spike S1 protein by ELISA. All other applications and species not yet tested.

Images

Figure 2 ELISA Validation of Gamma Variant Spike S1 Antibodies with SARS-CoV-2 Gamma Variant Spike S1 Protein Coating Antigen: SARS-CoV-2 spike S1 proteins WT and Gamma variant (P.1), 1 &956,g/mL, incubated at 4 &730,C overnight.Detection Antibodies: SARS-CoV-2 Spike P26S (Gamma Variant) antibody, PM-9590, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.SARS-CoV-2 Spike P26S (Gamma Variant) antibody specifically detects Gamma variant spike S1 protein, but not WT spike S1 protein (10-300).

Figure 4 ELISA Validation of Gamma Variant Spike S1 Antibodies with Mutant and WT Peptide Coating Antigen: SARS-CoV-2 spike S1 peptides: WT (9083P) and Gamma variant (P.1) (9573P), 1 &956,g/mL, incubated at 4 &730,C overnight.Detection Antibodies: SARS-CoV-2 26P antibody, PM-9590, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.SARS-CoV-2 Spike P26S (Gamma Variant) antibody detects Gamma variant spike S1 peptide (26S, 9573P), but not WT peptide (26P, 9083P).

Figure 1 SARS-Cov-2 Spike P26S (Gamma Variant) Antibodies Specifically Detect Gamma Variant Spike S1 Protein in an ELISA Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), mu variant (B.1.621), and omicron variant (B.1.1.529), 1 &956,g/mL, incubated at 4 &730,C overnight.Detection Antibodies: SARS-CoV-2 Spike P26S (Gamma Variant) antibody, dilution: 200 and 1000 ng/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.

Figure 3 WB Validation of Gamma Variant Spike S1 Antibodies with SARS-CoV-2 Gamma Variant Spike S1 Protein Loading: 50 ng of SARS-CoV-2 spike S1 proteins, including WT and Gamma variant (P.1).Detection Antibodies: Spike P26S (Gamma Variant) antibody, PM-9590, 1 &956,g/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.SARS-CoV-2 Spike P26S (Gamma Variant) antibody specifically detects Gamma variant spike S1 protein, but not WT spike S1 protein (10-300).